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Title: Salicylhydroxamic acid (SHAM) inhibition of the dissolved inorganic carbon concentrating process in unicellular green algae

Abstract

Rates of photosynthetic O{sub 2} evolution, for measuring K{sub 0.5}(CO{sub 2} + HCO{sub 3}{sup {minus}}) at pH 7, upon addition of 50 micromolar HCO{sub 3}{sup {minus}} to air-adapted Chlamydomonas, Dunaliella, or Scenedesmus cells, were inhibited up to 90% by the addition of 1.5 to 4.0 millimolar salicylhydroxamic acid (SHAM) to the aqueous medium. The apparent K{sub i}(SHAM) for Chlamydomonas cells was about 2.5 millimolar, but due to low solubility in water effective concentrations would be lower. Salicylhydroxamic acid did not inhibit oxygen evolution or accumulation of bicarbonate by Scenedesmus cells between pH 8 to 11 or by isolated intact chloroplasts from Dunaliella. Thus, salicylhydroxamic acid appears to inhibit CO{sub 2} uptake, whereas previous results indicate that vanadate inhibits bicarbonate uptake. These conclusions were confirmed by three test procedures with three air-adapted algae at pH 7. Salicylhydroxamic acid inhibited the cellular accumulation of dissolved inorganic carbon, the rate of photosynthetic O{sub 2} evolution dependent on low levels of dissolved inorganic carbon (50 micromolar NaHCO{sub 3}), and the rate of {sup 14}CO{sub 2} fixation with 100 micromolar ({sup 14}C)HCO{sub 3}{sup {minus}}. Salicylhydroxamic acid inhibition of O{sub 2} evolution and {sup 14}CO{sub 2}-fixation was reversed by higher levels of NaHCO{sub 3}. Thus, salicylhydroxamicmore » acid inhibition was apparently not affecting steps of photosynthesis other than CO{sub 2} accumulation. Although salicylhydroxamic acid is an inhibitor of alternative respiration in algae, it is not known whether the two processes are related.« less

Authors:
;  [1]
  1. Michigan State Univ., East Lansing (USA)
Publication Date:
OSTI Identifier:
5946378
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 92:3; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ORGANIC ACIDS; INHIBITION; UNICELLULAR ALGAE; PHOTOSYNTHESIS; CARBON 14 COMPOUNDS; CHLAMYDOMONAS; SCENEDESMUS; TRACER TECHNIQUES; ALGAE; CHEMICAL REACTIONS; CHLOROPHYCOTA; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; PHOTOCHEMICAL REACTIONS; PLANTS; SYNTHESIS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Goyal, A, and Tolbert, N E. Salicylhydroxamic acid (SHAM) inhibition of the dissolved inorganic carbon concentrating process in unicellular green algae. United States: N. p., 1990. Web. doi:10.1104/pp.92.3.630.
Goyal, A, & Tolbert, N E. Salicylhydroxamic acid (SHAM) inhibition of the dissolved inorganic carbon concentrating process in unicellular green algae. United States. https://doi.org/10.1104/pp.92.3.630
Goyal, A, and Tolbert, N E. 1990. "Salicylhydroxamic acid (SHAM) inhibition of the dissolved inorganic carbon concentrating process in unicellular green algae". United States. https://doi.org/10.1104/pp.92.3.630.
@article{osti_5946378,
title = {Salicylhydroxamic acid (SHAM) inhibition of the dissolved inorganic carbon concentrating process in unicellular green algae},
author = {Goyal, A and Tolbert, N E},
abstractNote = {Rates of photosynthetic O{sub 2} evolution, for measuring K{sub 0.5}(CO{sub 2} + HCO{sub 3}{sup {minus}}) at pH 7, upon addition of 50 micromolar HCO{sub 3}{sup {minus}} to air-adapted Chlamydomonas, Dunaliella, or Scenedesmus cells, were inhibited up to 90% by the addition of 1.5 to 4.0 millimolar salicylhydroxamic acid (SHAM) to the aqueous medium. The apparent K{sub i}(SHAM) for Chlamydomonas cells was about 2.5 millimolar, but due to low solubility in water effective concentrations would be lower. Salicylhydroxamic acid did not inhibit oxygen evolution or accumulation of bicarbonate by Scenedesmus cells between pH 8 to 11 or by isolated intact chloroplasts from Dunaliella. Thus, salicylhydroxamic acid appears to inhibit CO{sub 2} uptake, whereas previous results indicate that vanadate inhibits bicarbonate uptake. These conclusions were confirmed by three test procedures with three air-adapted algae at pH 7. Salicylhydroxamic acid inhibited the cellular accumulation of dissolved inorganic carbon, the rate of photosynthetic O{sub 2} evolution dependent on low levels of dissolved inorganic carbon (50 micromolar NaHCO{sub 3}), and the rate of {sup 14}CO{sub 2} fixation with 100 micromolar ({sup 14}C)HCO{sub 3}{sup {minus}}. Salicylhydroxamic acid inhibition of O{sub 2} evolution and {sup 14}CO{sub 2}-fixation was reversed by higher levels of NaHCO{sub 3}. Thus, salicylhydroxamic acid inhibition was apparently not affecting steps of photosynthesis other than CO{sub 2} accumulation. Although salicylhydroxamic acid is an inhibitor of alternative respiration in algae, it is not known whether the two processes are related.},
doi = {10.1104/pp.92.3.630},
url = {https://www.osti.gov/biblio/5946378}, journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 92:3,
place = {United States},
year = {Thu Mar 01 00:00:00 EST 1990},
month = {Thu Mar 01 00:00:00 EST 1990}
}