Evidence that isolated developing chloroplasts are capable of synthesizing chlorophyll b from 5-aminolevulinic acid

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.1.375· OSTI ID:5946149

Huang, Laiqiang; Hoffman, N E ^[1]

---

1. Carnegie Institution of Washington, Stanford, CA (USA)

Developing chloroplasts isolated from cucumber (Cucumis sativus L. var Beit Alpha) cotyledons are capable of incorporating ({sup 14}C)5-aminolevulinic acid into chlorophyll (Chl) b and Chl a when incubated under photosynthetic illumination. Thin layer chromatography and high pressure liquid chromatography were employed to analyze the pigments. The specific radioactivity in Chl a was over three times higher than that found in Chl b. Both Chl a and b synthesizing activities in organello decayed rapidly at approximately the same rate. We conclude that concomitant synthesis of Chl a/b-binding apoprotein is not required for Chl b synthesis.

• OSTI ID: 5946149
• Journal Information: Plant Physiology; (USA), Vol. 94:1; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINOLEVULINIC ACID
BIOCHEMICAL REACTION KINETICS
CHLOROPHYLL
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CHLOROPLASTS
CUCUMBERS
TRACER TECHNIQUES
AMINO ACIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
FOOD
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
PROTEINS
REACTION KINETICS
SYNTHESIS
VEGETABLES
550201* - Biochemistry- Tracer Techniques