Effect of thrombin and endotoxin on the in vivo metabolism of antithrombin III (AT III) in dogs
Effect of thrombin and endotoxin on the metabolism of I-125-labelled canine AT III was studied in mongrel dogs. Under control condition, mean total amount of intravascular AT III with standard deviation was 23.4 +/- 2.4 mg/kg, plasma half life of i.v. injected I-125-AT III was 1.7 +/- 0.2 days, and the fractional catabolic flux (j3x) was 16.3 +/- 1.6 mg/kg/day. The total amount of intra- and extra-vascular AT III was 36.0 +/- 0.34 mg/kg. Neither a 3 hour infusion of a small dose (30 units/kg/hr) of thrombin nor i.v. injection of a large amount of thrombin (5,000-15,000 units/day) with heparin significantly affected AT III metabolism except for a transient decrease in AT III concentration in the latter case, although decrease in plasma fibrinogen concentration and platelet count was observed in both cases. Two injections with 200 micrograms/kg of endotoxin resulted in an evident acceleration of AT III metabolism with significant decrease in the plasma AT III, fibrinogen concentrations and platelet count. More marked changes in AT III metabolism were induced by a single infusion with 1 mg/kg of endotoxin. Changes in hemostatic system coincided with those observed in DIC.
- Research Organization:
- Gunma Univ. School of Medicine, Japan
- OSTI ID:
- 5943472
- Journal Information:
- Thromb. Res.; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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ANTICOAGULANTS
METABOLISM
ENDOTOXINS
BIOLOGICAL EFFECTS
THROMBIN
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
DOGS
IODINE 125
LABELLED COMPOUNDS
MOLECULAR WEIGHT
TRACER TECHNIQUES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HYDROLASES
RADIOISOTOPES
REACTION KINETICS
SERINE PROTEINASES
TOXIC MATERIALS
TOXINS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics