In vitro study of cytotoxicity by U. V. radiation and differential sensitivity in combination with alkylating agents on established cell systems

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Cellular and Molecular Biology; (United States)

OSTI ID:5943404

Ramudu, K ^[1]

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1. Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad (India)

The effect of U.V. radiation or alkylating agents, such as actinomycin-D, cycloheximide and mitomycin-C (MMC), was studied on CHO, BHK and HeLa cells. U.V. radiation caused DNA ssb and dsb and were prevented by cycloheximide and actinomycin-D. MMC is known to be cytotoxic in CHO/BHK cells by forming free radical generation. MMC in combination with U.V. radiation enhanced DNA ssb dsb in these cell types. However, HeLa cells were insensitive to U.V. radiation. This insensitivity to U.V. radiation could be ascribed to the presence of glutathione transferase which is absent in CHO/BHK cell line.

• OSTI ID: 5943404
• Journal Information: Cellular and Molecular Biology; (United States), Vol. 37:2; ISSN 0145-5680
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
RADIOSENSITIVITY EFFECTS
ANIMAL CELLS
RADIOSENSITIVITY
ACTINOMYCIN
CHO CELLS
CYCLOHEXIMIDE
GENETIC VARIABILITY
HELA CELLS
MITOMYCIN
MUTAGENESIS
RADICALS
TRANSFERASES
ULTRAVIOLET RADIATION
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL VARIABILITY
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
FUNGICIDES
ORGANIC COMPOUNDS
PESTICIDES
PROTEINS
RADIATIONS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture