Radioiodinated, photoactivatable phosphatidylcholine and phosphatidylserine: transfer properties and differential photoreactive interaction with human erythrocyte membrane proteins
An isotopically labeled cross-linking reagent, succinimido 3-(3-(/sup 125/I)iodo-4-azidophenyl)propionate, has been synthesized and coupled to 1-acyl-2-(aminocaproyl)phosphatidylcholine according to previously described procedures. /sup 125/I- and N/sub 3/-labeled phosphatidylserine (/sup 125/I-N/sub 3/-PS) was produced from the phosphatidylcholine (PC) analog by phospholipase D catalyzed base exchange in the presence of L-serine. These phospholipid analogues are photoactivatable, are labeled with /sup 125/I at high specific activity, completely incorporate into synthetic vesicles, and spontaneously transfer between membranes. When an excess of acceptor vesicles or red blood cells (RBC) was mixed with a population of donor vesicles containing the /sup 125/I-N/sub 3/-phospholipids, approximately 40% of the analogues transferred to the acceptor population. After transfer in the dark to RBC, all of the /sup 125/I-N/sub 3/-PC incorporated into the cells could be removed by washing with serum, whereas the /sup 125/I-N/sub 3/-PS could not. After photolabeling of intact RBC, approx.50% of the PC and 20% of the PS cross-linked to membrane proteins as determined by their insolubility in CHCl/sub 3//MeOH. Analysis of probe distribution by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that /sup 125/I-N/sub 3/-PS preferentially labeled a M/sub r/ 30,000 peptide which contained approx.30% of the protein-bound label.
- Research Organization:
- Univ. of Texas M. D. Anderson Hospital and Tumor Institute, Houston
- OSTI ID:
- 5942594
- Journal Information:
- Biochemistry; (United States), Vol. 26:7
- Country of Publication:
- United States
- Language:
- English
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LABELLED COMPOUNDS
UPTAKE
PHOSPHOLIPIDS
AUTORADIOGRAPHY
CELL MEMBRANES
CHOLINE
ELECTROPHORESIS
ERYTHROCYTES
IODINE 125
ORGANIC IODINE COMPOUNDS
PEPTIDES
PHOTOCHEMISTRY
PROPIONIC ACID
SERINE
SODIUM IODIDES
ALCOHOLS
ALKALI METAL COMPOUNDS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
HALIDES
HALOGEN COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LIPIDS
LIPOTROPIC FACTORS
MATERIALS
MEMBRANES
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORS
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
SODIUM COMPOUNDS
550201* - Biochemistry- Tracer Techniques