Development of aquifer microcosms and in situ methods to test the fate and function of pollutant-degrading microorganisms
Our research goals for the past year were to: (1) validate the use of microcosms as predictors of microorganism survival in a polluted aquifer, and (2) develop sensitive methods for detecting the introduced microorganism, Pseudomonas sp. B13 (B13) a 3-chlorobenzoate degrader, from aquifer samples using molecular biology techniques, without the need for culturing. The microcosm and aquifer field studies were carried out at the US Geological Survey's Cape Cod study site at Camp Edwards in Falmouth, Massachusetts. Potential gene probes were developed at the German Institute for Biotechnology (GBF) and at Michigan State University (MSU). The Gene probes were evaluated for use in this project at MSU. The results suggest that microcosms could serve as models for short-term survival, but may not be reliable predictors of microbial fates for longer periods of time under some nutrient and redox conditions. Through the use of radiolabeled probes or internal primer sets we hope to improve the level of detection to near one organism per gram. 9 figs.
- Research Organization:
- Michigan State Univ., East Lansing, MI (USA). Dept. of Crop and Soil Sciences
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- FG02-89ER60848
- OSTI ID:
- 5941878
- Report Number(s):
- DOE/ER/60848-2; ON: DE91010748
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
54 ENVIRONMENTAL SCIENCES
AQUIFERS
MICROCOSMS
CHLORINATED AROMATIC HYDROCARBONS
BIODEGRADATION
MICROORGANISMS
POPULATION DYNAMICS
AQUATIC ECOSYSTEMS
BIOCHEMISTRY
DETECTION
ECOLOGY
GENETIC ENGINEERING
GROUND WATER
MASSACHUSETTS
PLUMES
POLLUTANTS
PROGRESS REPORT
PSEUDOMONAS
AROMATICS
BACTERIA
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DOCUMENT TYPES
ECOSYSTEMS
FEDERAL REGION I
HALOGENATED AROMATIC HYDROCARBONS
HYDROGEN COMPOUNDS
NORTH AMERICA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
USA
WATER
550700* - Microbiology
540320 - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)