Influence of bacterial toxins on the GTPase activity of transducin from bovine retinal rod outer segments
The action of cholera toxin, capable of ADP-ribosylation of the activator N/sub s/ protein, and pertussis toxin, capable of ADP-ribosylation of the inhibitor N/sub i/ protein of the adenylate cyclase complex, on transducin, the GTP-binding protein of the rod outer segments of the retina, was investigated. It was shown that under the action of pertussis and cholera toxins, the GTPase activity of transducin is inhibited. Pertussin toxin inhibits the GTPase of native retinal rod outer segments by 30-40%, while GTPase of homogeneous transducin produces a 70-80% inhibition. The action of toxins on transducin depends on the presence and nature of the guanylic nucleotide with which incubation is performed. On the basis of the data obtained it is suggested that pertussis toxin interacts with pretransducin and with the transducin-GDP complex, while cholera toxin ADP-ribosylates the transducin-GTP complex and does not act on transducin lacking GTP.
- Research Organization:
- Institute of Experimental Cardiology, Moscow, USSR
- OSTI ID:
- 5934062
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 50:11; Other Information: Translated from Biokhimiya; 50: No. 11, 1825-1835(Nov 1985)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of transducin from bovine retinal rod outer segments: mechanism and effects of cholera toxin-catalyzed adp-ribosylation
Regulation of follitropin-sensitive adenylate cyclase by stimulatory and inhibitory forms of the guanine nucleotide regulatory protein in immature rat Sertoli cells
Related Subjects
BACTERIA
BIOLOGICAL EFFECTS
PROTEINS
ENZYME ACTIVITY
RADIOENZYMATIC ASSAY
TOXINS
BIOCHEMISTRY
CATTLE
CHOLERA
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYME INHIBITORS
ENZYMES
GUANOSINE
INHIBITION
LABELLED COMPOUNDS
METABOLIC ACTIVATION
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
RETINA
SUBSTRATES
ANIMALS
ANTIGENS
AROMATICS
AZAARENES
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BODY AREAS
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DISEASES
DOMESTIC ANIMALS
EYES
FACE
HEAD
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LYSIS
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PURINES
RADIOISOTOPES
RIBOSIDES
RUMINANTS
SENSE ORGANS
SOLVOLYSIS
TOXIC MATERIALS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques