Use of (2-/sup 14/C)mevalonate and saponin-bound (/sup 14/C)-3-hydroxy-3-methylglutaric acid for the biosynthesis of terpenoids in leaves of Dioscorea deltoidea
After the introduction of (2-/sup 14/C)acetate into leaves of Dioscorea deltoidea, a radioactive furonanalog of deltafolin - protodeltofolin, containing two-thirds of the label in the 3-hydroxy-3-methylglutaryl portion - was isolated from them. Radioactive ..beta..-careotene and sterols were isolated from cut young leaves of Dioscorea 24 h after the introduction of (/sup 14/C) protodeltofolin into them, using chromatography on a column of silica gel and precipitation of sterols in the form of digitonins for this purpose. The incorporation of radioactivity from (/sup 14/C)-3-hydroxy-3-methyl-glutaric acid, bound in the form of a saponin, and ..beta..-carotene came to 0.18-0.80%, while incorporation into sterols came to 0.07-2.86% of the radioactivity of the alcohol extract. Thereby it was shown that 3-hydroxyl-3-methylglutaric acid, bound in the form of the saponin, can be used to form terpenoids in Dioscorea leaves. It was suggested that the binding of hydroxymethylglutaric acid to saponin represents one of the mechanisms of regulation of the rate of terpenoid biosynthesis in Dioscorea leaves.
- Research Organization:
- A.N. Bakh Institute of Biochemistry, Moscow, USSR
- OSTI ID:
- 5934047
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 50:9; Other Information: Translated from Biokhimiya; 50: No. 9, 1493-1498(Sep 1985)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXYLIC ACID ESTERS
BIOCHEMISTRY
CARBOXYLIC ACID SALTS
LEAVES
RADIORECEPTOR ASSAY
SAPONINS
TERPENES
BIOSYNTHESIS
ACETATES
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CAROTENOIDS
CHROMATOGRAPHY
GLUTARIC ACID
MEVALONIC ACID
STEROLS
UPTAKE
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMISTRY
DICARBOXYLIC ACIDS
ESTERS
GLYCOSIDES
HYDROCARBONS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PIGMENTS
REACTION KINETICS
SEPARATION PROCESSES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques