Correlation of histology and drug response of human tumors grown in native-state three-dimensional histoculture and in nude mice

Journal Article · Sat Jun 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.12.5163· OSTI ID:5933688

Vescio, R A; Connors, K M ^[1]; Kubota, Tetsuro ^[2]; Hoffman, R M ^[3]

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1. AntiCancer, Inc., San Diego, CA (United States)
2. Keio Univ., Tokyo (Japan)
3. AntiCancer, Inc., San Diego, CA (United States) Univ. of California, San Diego, La Jolla, CA (United States)

An in vitro histoculture system in which a native-state collagen-sponge gel supports the three-dimensional growth of tumor tissue has been recently developed that allows the culture and drug response assay for most every tumor type. Important features of the histoculture system include the maintenance of three-dimensional tissue architecture and the use of histological autoradiography to measure nuclear incorporation of ({sup 3}H)thymidine as an endpoint. The authors describe in this report in vitro-in vivo correlations for drug response and tumor histology by using human tumor xenografts grown in the native-state three-dimensional histoculture system and as xenografts in nude mice. This comparison eliminates many of the confounding variables seen in most correlative clinical trials. Results demonstrate (1) a very high preservation of in vivo tissue architecture in vitro, (2) an 86% accuracy in vitro of predicting drug resistance in vivo, and (3) an overall predictive frequency of drug resistance and sensitivity ranging from 53% for 5-fluorouracil to 78% for doxorubicin.

• OSTI ID: 5933688
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:12; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DOXORUBICIN
TOXICITY
FLUOROURACILS
NEOPLASMS
AUTORADIOGRAPHY
CHEMOTHERAPY
CYTOLOGICAL TECHNIQUES
HISTOLOGY
MAN
THYMIDINE
TISSUE CULTURES
TRITIUM COMPOUNDS
UPTAKE
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMETABOLITES
ANTINEOPLASTIC DRUGS
AZINES
DISEASES
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RIBOSIDES
THERAPY
URACILS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques