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Title: Incorporation of oxygen into abscisic acid and phaseic acid for molecular oxygen

Abstract

Abscisic acid accumulates in detached, wilted leaves of Xanthium strumariu. When these leaves are subsequently rehydrated, phaseic acid, a catabolite of abscisic acid, accumulates. Analysis by gas chromatography-mass spectrometry of phaseic acid isolated from stressed and subsequently rehydrated leaves placed in an atmosphere containing 20% /sup 18/O/sub 2/ and 80% N/sub 2/ indicates that one atom of /sup 18/O is incorporated in the 6'-hydroxymethyl group of phaseic acid. This suggests that the enzyme that converts abscisic acid to phaseic acid is an oxygenase. Analysis by gas chromatography-mass spectrometry of abscisic acid isolated from stressed leaves kept in an atmosphere containing /sup 18/O/sub 2/ indicates that one atom of /sup 18/O is presented in the carboxyl group of abscisic acid. Thus, when abscisic acid accumulates in water-streesed leaves, only one of the four oxygens present in the abscisic acid molecule is derived from molecular oxygen. This suggest that either (a) the oxygen present in the 1'-, 4'-, and one of the two oxygens at the 1-position of abscisic acid arise from water, or (b) there exists a stored precursor with oxygen atoms already present in the 1'- and 4'-positions of abscisic acid which is converted to abscisic acid under conditions ofmore » water stress. 17 references, 2 figures, 1 tables.« less

Authors:
;
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States)
OSTI Identifier:
5932422
DOE Contract Number:  
AC02-76ER01338
Resource Type:
Journal Article
Journal Name:
Plant Physiol.; (United States)
Additional Journal Information:
Journal Volume: 75:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ORGANIC ACIDS; BIOCHEMISTRY; BIOLOGICAL ACCUMULATION; BIOLOGICAL STRESS; CHEMICAL ANALYSIS; ENZYME ACTIVITY; GAS CHROMATOGRAPHY; HERBS; LEAVES; MASS SPECTROSCOPY; OXYGEN 18; OXYGENASES; WATER; CHEMISTRY; CHROMATOGRAPHY; ENZYMES; EVEN-EVEN NUCLEI; HYDROGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; NUCLEI; ORGANIC COMPOUNDS; OXIDOREDUCTASES; OXYGEN COMPOUNDS; OXYGEN ISOTOPES; PLANTS; SEPARATION PROCESSES; SPECTROSCOPY; STABLE ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Creelman, R A, and Zeevaart, J A.D. Incorporation of oxygen into abscisic acid and phaseic acid for molecular oxygen. United States: N. p., 1984. Web. doi:10.1104/pp.75.1.166.
Creelman, R A, & Zeevaart, J A.D. Incorporation of oxygen into abscisic acid and phaseic acid for molecular oxygen. United States. https://doi.org/10.1104/pp.75.1.166
Creelman, R A, and Zeevaart, J A.D. 1984. "Incorporation of oxygen into abscisic acid and phaseic acid for molecular oxygen". United States. https://doi.org/10.1104/pp.75.1.166.
@article{osti_5932422,
title = {Incorporation of oxygen into abscisic acid and phaseic acid for molecular oxygen},
author = {Creelman, R A and Zeevaart, J A.D.},
abstractNote = {Abscisic acid accumulates in detached, wilted leaves of Xanthium strumariu. When these leaves are subsequently rehydrated, phaseic acid, a catabolite of abscisic acid, accumulates. Analysis by gas chromatography-mass spectrometry of phaseic acid isolated from stressed and subsequently rehydrated leaves placed in an atmosphere containing 20% /sup 18/O/sub 2/ and 80% N/sub 2/ indicates that one atom of /sup 18/O is incorporated in the 6'-hydroxymethyl group of phaseic acid. This suggests that the enzyme that converts abscisic acid to phaseic acid is an oxygenase. Analysis by gas chromatography-mass spectrometry of abscisic acid isolated from stressed leaves kept in an atmosphere containing /sup 18/O/sub 2/ indicates that one atom of /sup 18/O is presented in the carboxyl group of abscisic acid. Thus, when abscisic acid accumulates in water-streesed leaves, only one of the four oxygens present in the abscisic acid molecule is derived from molecular oxygen. This suggest that either (a) the oxygen present in the 1'-, 4'-, and one of the two oxygens at the 1-position of abscisic acid arise from water, or (b) there exists a stored precursor with oxygen atoms already present in the 1'- and 4'-positions of abscisic acid which is converted to abscisic acid under conditions of water stress. 17 references, 2 figures, 1 tables.},
doi = {10.1104/pp.75.1.166},
url = {https://www.osti.gov/biblio/5932422}, journal = {Plant Physiol.; (United States)},
number = ,
volume = 75:1,
place = {United States},
year = {Tue May 01 00:00:00 EDT 1984},
month = {Tue May 01 00:00:00 EDT 1984}
}