Sex and strain differences in the hepatocyte primary culture/DNA repair test

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850180205· OSTI ID:5929478

McQueen, C A; Way, B M ^[1]

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1. American Health Foundation, Valhalla, NY (United States)

The hepatocyte primary culture (HPC)/DNA repair test was developed using hepatocytes isolated from male F-344 rats. A number of genetic polymorphisms have been shown to occur in inbred strains of rats, which may lead to variation in biotransformation of xenobiotics resulting in differences in susceptibility to genotoxins. The effect of the strain utilized as a source of hepatocytes was investigated with female Lewis, F-344, and DA rats. Variation was observed when hepatocytes from the three strains were exposed to aflatoxin B{sub 1} (AFB{sub 1}). No clearcut strain differences were seen when cells were exposed to diethylnitrosamine (DEN) or 2-acetylaminofluorene. These results demonstrate that both the strain and the sex of the animal used as a source of hepatocytes can affect the HPC/DNA repair test.

• OSTI ID: 5929478
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Vol. 18:2; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
GENETIC EFFECTS
AFLATOXINS
LIVER CELLS
DNA REPAIR
NITROSAMINES
CELL CULTURES
RATS
SEX DEPENDENCE
XENOBIOTICS
AMINES
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
MAMMALS
MATERIALS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology