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Title: Potassium-dependent changes in the expression of membrane-associated proteins in barley roots

Abstract

Barley (Hordeum vulgare L. cv Halcyon) seedlings which has been grown in full strength complete inorganic nutrient media (containing 6 millimolar K{sup +}) had high internal K{sup +} concentrations and low values of K{sup +} ({sup 86}Rb{sup +}) influx when influx was measured from solutions containing 100 micromolar K{sup +}. Transfer of these plants to solutions lacking K{sup +} resulted in significant reductions of root and shoot K{sup +} concentrations and values of K{sup +} ({sup 86}Rb{sup +}) influx increased by greater than 10-fold within 3 days. When plants treated in this way were returned to complete solutions, containing K{sup +}, the changes induced by K{sup +} deprivation were reversed. Parallel studies of microsomal membranes by means of SDS-PAGE demonstrated that the expression of a group of polypeptides increased or decreased in parallel with changes of K{sup +} ({sup 86}Rb{sup +}) influx. Most prominent of these were 45 and 34 kilodalton polypeptides which specifically responded to K{sup +} status of the barley plants; their expression was not enhanced by N or P deprivation. The 45 kilodalton polypeptide was susceptible to degradation by a membrane associated protease when microsomes were washing in buffer containing 0.2 millimolar PMSF. This loss was preventedmore » by increasing PMSF concentration to 2 millimolar.« less

Authors:
; ; ;  [1]
  1. Univ. of British Columbia, Vancouver (Canada)
Publication Date:
OSTI Identifier:
5923338
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 92:4; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; POTASSIUM; BIOLOGICAL EFFECTS; BARLEY; MEMBRANE PROTEINS; POLYPEPTIDES; ROOTS; RUBIDIUM 86; RUBIDIUM COMPOUNDS; SEEDLINGS; TRACER TECHNIQUES; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; ALKALI METALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CEREALS; DAYS LIVING RADIOISOTOPES; ELEMENTS; GRASS; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LILIOPSIDA; MAGNOLIOPHYTA; METALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PEPTIDES; PLANTS; PROTEINS; RADIOISOTOPES; RUBIDIUM ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Fernando, M, Kulpa, J, Siddiqi, M Y, and Glass, A D.M. Potassium-dependent changes in the expression of membrane-associated proteins in barley roots. United States: N. p., 1990. Web. doi:10.1104/pp.92.4.1128.
Fernando, M, Kulpa, J, Siddiqi, M Y, & Glass, A D.M. Potassium-dependent changes in the expression of membrane-associated proteins in barley roots. United States. https://doi.org/10.1104/pp.92.4.1128
Fernando, M, Kulpa, J, Siddiqi, M Y, and Glass, A D.M. 1990. "Potassium-dependent changes in the expression of membrane-associated proteins in barley roots". United States. https://doi.org/10.1104/pp.92.4.1128.
@article{osti_5923338,
title = {Potassium-dependent changes in the expression of membrane-associated proteins in barley roots},
author = {Fernando, M and Kulpa, J and Siddiqi, M Y and Glass, A D.M.},
abstractNote = {Barley (Hordeum vulgare L. cv Halcyon) seedlings which has been grown in full strength complete inorganic nutrient media (containing 6 millimolar K{sup +}) had high internal K{sup +} concentrations and low values of K{sup +} ({sup 86}Rb{sup +}) influx when influx was measured from solutions containing 100 micromolar K{sup +}. Transfer of these plants to solutions lacking K{sup +} resulted in significant reductions of root and shoot K{sup +} concentrations and values of K{sup +} ({sup 86}Rb{sup +}) influx increased by greater than 10-fold within 3 days. When plants treated in this way were returned to complete solutions, containing K{sup +}, the changes induced by K{sup +} deprivation were reversed. Parallel studies of microsomal membranes by means of SDS-PAGE demonstrated that the expression of a group of polypeptides increased or decreased in parallel with changes of K{sup +} ({sup 86}Rb{sup +}) influx. Most prominent of these were 45 and 34 kilodalton polypeptides which specifically responded to K{sup +} status of the barley plants; their expression was not enhanced by N or P deprivation. The 45 kilodalton polypeptide was susceptible to degradation by a membrane associated protease when microsomes were washing in buffer containing 0.2 millimolar PMSF. This loss was prevented by increasing PMSF concentration to 2 millimolar.},
doi = {10.1104/pp.92.4.1128},
url = {https://www.osti.gov/biblio/5923338}, journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 92:4,
place = {United States},
year = {Sun Apr 01 00:00:00 EST 1990},
month = {Sun Apr 01 00:00:00 EST 1990}
}