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Title: Glycolate metabolism in low and high CO sub 2 -grown chlorella pyrenoidosa and Pavlova lutheri as determined by sup 18 O-labeling

Abstract

Photorespiration in Chlorella pyrenoidosa Chick. was assayed by measuring {sup 18}O-labeled intermediates of the glycolate pathway. Glycolate, glycine, serine, and excreted glycolate were isolated and analyzed on a gas chromatograph/mass spectrometer to determine isotopic enrichment. Rates of glycolate synthesis were determined from {sup 18}O-labeling kinetics of the intermediates, pool sizes, derived rate equations, and nonlinear regression techniques. Glycolate synthesis was higher in high CO{sub 2}-grown cells than in air-grown cells when both were assayed under the same O{sub 2} and CO{sub 2} concentrations. Synthesis of glycolate, for both types of cells, was stimulated by high O{sub 2} levels and inhibited by high CO{sub 2} levels. Glycolate synthesis in 1.5% CO{sub 2}-grown Chlorella, when exposed to a 0.035% CO{sub 2} atmosphere, increased from about 41 to 86 nanomoles per milligram chlorophyll per minute when the O{sub 2} concentration was increased from 21 to 40%. Glycolate synthesis in air-grown cells increased from 2 to 6 nanomoles per milligram chlorophyll per minute under the same gas levels. Synthesis was undetectable when either the O{sub 2} concentration was lowered to 2% or the CO{sub 2}-concentration was raised to 1.5%. Glycolate excretion was also sensitive to O{sub 2} and CO{sub 2} concentrations in 1.5% CO{submore » 2}-grown cells and the glycolate that was excreted was {sup 18}O-labeled. Air-grown cells did not excrete glycolate under any experimental condition. Indirect evidence indicated that glycolate may be excreted as a lactone in Chlorella. Photorespiratory {sup 18}O-labeling kinetics were determined for Pavlova lutheri, which unlike Chlorella and higher plants did not directly synthesize glycine and serine from glycolate. This alga did excrete a significant proportion of newly synthesized glycolate into the media.« less

Authors:
;  [1]
  1. Pennsylvania State Univ., University Park (USA)
Publication Date:
OSTI Identifier:
5922423
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 91:3; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; CARBON DIOXIDE; BIOLOGICAL EFFECTS; CHLORELLA; GLYCOLIC ACID; METABOLISM; OXYGEN 18; OXYGEN COMPOUNDS; TRACER TECHNIQUES; ALGAE; CARBON COMPOUNDS; CARBON OXIDES; CARBOXYLIC ACIDS; CHALCOGENIDES; CHLOROPHYCOTA; EVEN-EVEN NUCLEI; HYDROXY ACIDS; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; MONOCARBOXYLIC ACIDS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXIDES; OXYGEN ISOTOPES; PLANTS; STABLE ISOTOPES; UNICELLULAR ALGAE; 550201* - Biochemistry- Tracer Techniques; 540110

Citation Formats

de Veau, E J, and Burris, J E. Glycolate metabolism in low and high CO sub 2 -grown chlorella pyrenoidosa and Pavlova lutheri as determined by sup 18 O-labeling. United States: N. p., 1989. Web. doi:10.1104/pp.91.3.1085.
de Veau, E J, & Burris, J E. Glycolate metabolism in low and high CO sub 2 -grown chlorella pyrenoidosa and Pavlova lutheri as determined by sup 18 O-labeling. United States. https://doi.org/10.1104/pp.91.3.1085
de Veau, E J, and Burris, J E. 1989. "Glycolate metabolism in low and high CO sub 2 -grown chlorella pyrenoidosa and Pavlova lutheri as determined by sup 18 O-labeling". United States. https://doi.org/10.1104/pp.91.3.1085.
@article{osti_5922423,
title = {Glycolate metabolism in low and high CO sub 2 -grown chlorella pyrenoidosa and Pavlova lutheri as determined by sup 18 O-labeling},
author = {de Veau, E J and Burris, J E},
abstractNote = {Photorespiration in Chlorella pyrenoidosa Chick. was assayed by measuring {sup 18}O-labeled intermediates of the glycolate pathway. Glycolate, glycine, serine, and excreted glycolate were isolated and analyzed on a gas chromatograph/mass spectrometer to determine isotopic enrichment. Rates of glycolate synthesis were determined from {sup 18}O-labeling kinetics of the intermediates, pool sizes, derived rate equations, and nonlinear regression techniques. Glycolate synthesis was higher in high CO{sub 2}-grown cells than in air-grown cells when both were assayed under the same O{sub 2} and CO{sub 2} concentrations. Synthesis of glycolate, for both types of cells, was stimulated by high O{sub 2} levels and inhibited by high CO{sub 2} levels. Glycolate synthesis in 1.5% CO{sub 2}-grown Chlorella, when exposed to a 0.035% CO{sub 2} atmosphere, increased from about 41 to 86 nanomoles per milligram chlorophyll per minute when the O{sub 2} concentration was increased from 21 to 40%. Glycolate synthesis in air-grown cells increased from 2 to 6 nanomoles per milligram chlorophyll per minute under the same gas levels. Synthesis was undetectable when either the O{sub 2} concentration was lowered to 2% or the CO{sub 2}-concentration was raised to 1.5%. Glycolate excretion was also sensitive to O{sub 2} and CO{sub 2} concentrations in 1.5% CO{sub 2}-grown cells and the glycolate that was excreted was {sup 18}O-labeled. Air-grown cells did not excrete glycolate under any experimental condition. Indirect evidence indicated that glycolate may be excreted as a lactone in Chlorella. Photorespiratory {sup 18}O-labeling kinetics were determined for Pavlova lutheri, which unlike Chlorella and higher plants did not directly synthesize glycine and serine from glycolate. This alga did excrete a significant proportion of newly synthesized glycolate into the media.},
doi = {10.1104/pp.91.3.1085},
url = {https://www.osti.gov/biblio/5922423}, journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 91:3,
place = {United States},
year = {Wed Nov 01 00:00:00 EST 1989},
month = {Wed Nov 01 00:00:00 EST 1989}
}