Sequential sup 1 H NMR assignments and secondary structure of the B domain of staphylococcal protein A: Structural changes between the free B domain in solution and the Fc-bound B domain in crystal
- Univ. of Tokyo (Japan)
- Kyowa Hakko Kogyo Co., Asahimachi, Tokyo (Japan)
The recombinant B domain (FB) of staphylococcal protein A, which specifically binds to the Fc portion of immunoglobulin G (IgG), has been investigated with the use of two-dimensional proton nuclear magnetic resonance spectroscopy. All backbone and side-chain proton resonances of FB (60 amino acid residues), except the amide proton resonance of Ala2, were assigned by the sequential assignment procedures by using double-quantum-filtered correlated spectroscopy (DQF-COSY), homonuclear Hartmann-Hahn spectroscopy (HOHAHA), and nuclear Overhauser enhancement spectroscopy (NOESY). On the basis of the NOESY data, three helical regions, Glu9-His19, Glu25-Asp37, and Ser42-Ala55, were identified in the free FB in solution. Existence of two of the three helical regions, Glu9-His19 and Glu25-Asp37, is consistent with the X-ray crystallographic structure of the Fc-bound FB. By contrast, in the Fc-bound FB as revealed by the X-ray analysis, the Ser42-Glu48 segment and no structural information has been available in the Ala49-Ala55 segment. The authors suggest that a significant conformation change is induced in the C-terminal region of FB when it is bound to the Fc portion of IgG.
- OSTI ID:
- 5920959
- Journal Information:
- Biochemistry; (USA), Vol. 29:37; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
NUCLEAR MAGNETIC RESONANCE
CELL WALL
CHEMICAL SHIFT
CONFORMATIONAL CHANGES
GLUTAMIC ACID
HISTIDINE
OVERHAUSER EFFECT
PROTONS
SERINE
STAPHYLOCOCCUS
TEMPERATURE DEPENDENCE
AMINO ACIDS
AZOLES
BACTERIA
BARYONS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
IMIDAZOLES
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RESONANCE
550601* - Medicine- Unsealed Radionuclides in Diagnostics