Fermentation method producing ethanol

Patent · Tue Feb 04 00:00:00 EST 1986

OSTI ID:5918286

Wang, D I.C.; Dalal, R

This patent describes a process for preparing and isolating a mutant strain of Clostridium thermosaccharolyticum. The mutant strain is able to ferment hexose and pentose carbohydrates to produce ethanol and acetic acid in gram ratios of at least about 8:1. The process includes the steps of: 1.) exposing Clostridium thermosaccharolyticum cells to a mutagenic agent sufficient to effect mutation of the cells; 2.) culturing the mutated cells in a growth medium containing minimal carbon sources and pyruvate for a predetermined time period; 3.) enriching the growth medium with at least one antibiotic, the antibiotic killing the actively growing cells in the medium without substantially affecting the non-actively growing cells; and 4.) isolating a mutant Clostridium thermosaccharolyticium strain from the non-actively growing cells via the inability to utilize pyruvate as a carbon source.

• Assignee: Massachusetts Institute of Tech., Cambridge, MA
• Patent Number(s): US 4568644
• OSTI ID: 5918286
• Resource Relation: Patent File Date: Filed date 13 Aug 1984
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
BIOSYNTHESIS
ETHANOL
HEXOSES
FERMENTATION
PENTOSES
ANTIBIOTICS
CARBON
CLOSTRIDIUM THERMOSACCHAROLYTICUM
CULTURE MEDIA
MUTAGENESIS
MUTANTS
PYRUVIC ACID
ALCOHOLS
ANTI-INFECTIVE AGENTS
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CARBOXYLIC ACIDS
CLOSTRIDIUM
DRUGS
ELEMENTS
HYDROXY COMPOUNDS
KETO ACIDS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SACCHARIDES
SYNTHESIS
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