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Title: Natural killer (NK) activity of pit cells perfused from livers of rats treated with ethanol

The liver is the major site of ethanol (ETOH) metabolism. Liver sinusoids contain lymphocytes with NK activity. The authors treated LEW rats for 2 weeks with i.p. injection of 1.25 ml 25% ETOH/kg 3 times/week and 5% ETOH in drinking water. Livers were perfused at 5-fold physiological pressure and cells obtained were banded on 1.077 density Ficoll. Their cytotoxicity was tested against {sup 51}Cr-labeled YAC-1 or U937 and compared to spleen and blood lymphocytes. In untreated rats, pit cell NK activity was 2-fold that of splenic lymphocytes and 4-fold that of blood lymphocytes. Compared to controls, ETOH-treated rats exhibited a 30 to 90% rise in pit cell NK activity detected with YAC-1 or U937 targets. The pit cell enhanced NK activity in ETOH-treated rats was further increased if polyinosinicpolycytidilic acid was injection i.p. 18 hours before the assay. Blood and spleen lymphocyte NK activity of ETOH-treated rats was also greater than in controls. There was no evidence that ETOH merely redistributed lymphocytes among the tissues. Although ETOH acutely inhibits NK activity in vitro, chronic ETOH increases in vivo.
Authors:
; ; ;  [1]
  1. (National Inst. of Alcohol Abuse and Alcoholism, Rockville, MD (United States) Univ. of Arkansas Medical Sciences, Little Rock (United States))
Publication Date:
OSTI Identifier:
5905513
Report Number(s):
CONF-9104107--
Journal ID: ISSN 0892-6638; CODEN: FAJOE
Resource Type:
Conference
Resource Relation:
Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States); Journal Volume: 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ETHANOL; BIOLOGICAL EFFECTS; LYMPHOCYTES; CELL PROLIFERATION; BLOOD; CHROMIUM 51; LIVER; METABOLISM; PERFUSED ORGANS; RATS; SPLEEN CELLS; TRACER TECHNIQUES; ALCOHOLS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY; BODY FLUIDS; CHROMIUM ISOTOPES; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; GLANDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology