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Title: Comparative effects of glyphosate and atrazine in chloroplast ultrastructure of wheat and downy brome. [Triticum aestivum; Bromus tectorum]

Developing and mature leaves of winter wheat (Triticum aestivum L. var. Daws) and the weed species downy brome (Bromus tectorum L.) were subjected to 10 mM (foliar application) and 1 mM (root application) herbicide solutions. Glyphosate (N-(phosphonomethyl) glycine) and atrazine (2-chloro-4-(ethyl-amino)-6-(isopropylamino)-s-triazine) were prepared in a carrier composed of 5% soybean oil concentrate, 35% acetone and 60% water. Penetration experiments with /sup 3/H-labelled herbicides assessed what percentage of herbicide entered leaves, and microautoradiography was used to determine qualitatively how much herbicide was present in the sections viewed with TEM. Tissue was excised at 4, 18, 62 and 200 hours, and then either freeze-substituted or fixed chemically. Ultrastructural effects of each herbicide on chloroplasts from leaves of newly-germinated seedlings and of well-tillered plants are depicted and discussed. Temporal differences in response of chloroplasts to each herbicide are noted.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
5897337
Report Number(s):
CONF-8707108-
Journal ID: CODEN: PPYSA; TRN: 87-037639
Resource Type:
Conference
Resource Relation:
Journal Name: Plant Physiol., Suppl.; (United States); Journal Volume: 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
Research Org:
Dept. of Agriculture-ARS, Pullman, WA
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 60 APPLIED LIFE SCIENCES; HERBICIDES; ABSORPTION; BIOLOGICAL EFFECTS; AUTORADIOGRAPHY; CHLOROPLASTS; LEAVES; ROOT ABSORPTION; SEEDLINGS; TRACER TECHNIQUES; TRANSMISSION ELECTRON MICROSCOPY; TRITIUM COMPOUNDS; WEEDS; WHEAT; CELL CONSTITUENTS; CEREALS; ELECTRON MICROSCOPY; GRASS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROSCOPY; PESTICIDES; PLANTS; UPTAKE 560300* -- Chemicals Metabolism & Toxicology; 553006 -- Agriculture & Food Technology-- Other Agricultural Applications-- (1987-)