Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate

Journal Article · Sat Jun 01 00:00:00 EDT 1996 · Biotechnology and Applied Biochemistry

OSTI ID:588677

Raugei, G; Modesti, A; Magherini, F ^[1]

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1. Universita di Firenze (Italy); and others

Previous experiments in vitro have demonstrated the ability of acylphosphatase to increase the rate of glucose fermentation in yeast. To evaluate the possibility of increasing fermentation in vivo also, a chemically synthesized DNA sequence coding for human muscle acylphosphatase was expressed at high level in Saccharomyces cerevisiae. Ethanol production was measured in these engineered strains in comparison with a control. Acylphosphatase expression strongly increased the rate of ethanol production both in aerobic and anaerobic culture. This finding may be potentially important for the development of more efficient industrial fermentation processes. 20 refs., 5 figs.

• OSTI ID: 588677
• Journal Information: Biotechnology and Applied Biochemistry, Vol. 23, Issue 3; Other Information: PBD: Jun 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
09 BIOMASS FUELS
32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
ETHANOL
FERMENTATION
GLUCOSE
PRODUCTION
SACCHAROMYCES CEREVISIAE
ACYL RADICALS
DNA SEQUENCING
GENE REGULATION
DNA
GENETIC ENGINEERING
AEROBIC CONDITIONS
ANAEROBIC CONDITIONS
BIOTECHNOLOGY
ENZYME ACTIVITY
PLASMIDS