Morphologic and phenotypic analysis of an outcross line of blotchy mouse

Journal Article · Sat Jan 01 00:00:00 EST 1983 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148309055014· OSTI ID:5882798

Ranga, V; Grahn, D; Journey, T M

Blotchy is an X-linked recessive mutation at the Mottled locus in the mouse. The affected blotchy male (Blo/Y) mouse from an inbred genetic background demonstrates morphologic and physiologic abnormalities consistent with emphysema in adult life. Breeding of Blo/Y mice has been difficult because the inbred Blo/Y males are sterile. We report the successful development of a line of outbred Blo/Y male and Blo/Blo female mice by the controlled outcross mating of the inbred heterozygous Blo/ + female with the Argonne hybrid B6CF/sub 1/ male mouse. The subsequent outcross Blo/Y progeny breed vigorously with outcrossed Blo/ + female. The lungs of the outbred Blo/Blo female and inbred Blo/Y male mice demonstrate mild to moderate panacinar emphysema with a significant decrease in internal surface area (p < 0.005) and an increase in mean linear intercept (p < 0.005). In contrast, the lungs of the outbred Blo/Y is structurally normal. Despite the absence of emphysema-like changes in the outbred Blo/Y males, there were penotypic features that suggest inherited abnormalities in connective tissue proteins including (1) high incidence of aortitis leading to premature death from aneurysmal rupture, and (2) significant decrease in the morphometrically determined parenchymal elastic fiber length in the lung (p < 0.01). The outbred blotchy strain may be a useful experimental animal model in determining the pathogenesis of emphysema. 21 references, 1 figure, 3 tables.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5882798
• Journal Information: Exp. Lung Res.; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EMPHYSEMA
BIOLOGICAL MODELS
PATHOGENESIS
MICE
RECESSIVE MUTATIONS
ANIMAL BREEDING
CROSS-LINKING
EXPERIMENTAL DATA
GENETICS
HEREDITARY DISEASES
LUNGS
MORPHOLOGY
PHENOTYPE
PROGENY
ANIMALS
BIOLOGY
BODY
CHEMICAL REACTIONS
DATA
DISEASES
INFORMATION
MAMMALS
MUTATIONS
NUMERICAL DATA
ORGANS
POLYMERIZATION
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
VERTEBRATES
550400* - Genetics