Polyacrylamide gel miniaturization improves protein visualization and autoradiographic detection

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(89)90053-5· OSTI ID:5879328

Mohamed, M A; Lerro, K A; Prestwich, G D

Polyacrylamide gels shrink to one-quarter of their original area when soaked in a 50% (w/v) solution of polyethylene glycol. Gel miniaturization improves the contrast of protein bands, with four valuable consequences. (i) A 5- to 10-fold increase in sensitivity for Coomassie blue is observed. (ii) Gels are more durable; i.e., they resist tearing when wet and they do not crack during drying under vacuum. (iii) Shrunken gels give sharper photographic images and provide better interlane protein band comparisons. (iv) Condensed protein bands lead to an increased sensitivity for detecting low-abundance, radioactively-labeled proteins by fluorography.

• Research Organization: State Univ. of New York, Stony Brook (USA)
• OSTI ID: 5879328
• Journal Information: Anal. Biochem.; (United States), Vol. 177:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
AUTORADIOGRAPHY
AFFINITY
ELECTROPHORESIS
INSECTS
LABELLED COMPOUNDS
MOTHS
OPTIMIZATION
POLYETHYLENE GLYCOLS
RADIOISOTOPES
ALCOHOLS
ANIMALS
ARTHROPODS
GLYCOLS
HYDROXY COMPOUNDS
INVERTEBRATES
ISOTOPES
LEPIDOPTERA
ORGANIC COMPOUNDS
ORGANIC POLYMERS
POLYMERS
550201* - Biochemistry- Tracer Techniques