Wound-induced changes in mRNA populations in tomato pericarp tissue
Immature green tomato pericarp tissue was wounded by cutting into small pieces. At various intervals, ethylene production was monitored and the corresponding tissue harvested for mRNA extraction. Poly (A)/sup +/ RNA was fractionated from total RNA using oligo (dT)-cellulose chromatography and was translated in vitro in a rabbit reticulocyte lysate system using /sup 35/S-methionine. Labeled products were subjected to one and two dimensional polyacrylamide gel electrophoresis (PAGE) to analyze wound-induced changes in mRNA populations. Analyses of autoradiograms of corresponding single dimension SDS-PAGE showed changes in at least 12 major polypeptides with 6 declining (18, 19, 24, 36, 44, 69 kD) and 6 increasing (21, 41, 46, 54, 75, > 94 kD) after wounding. Among the polypeptides resolved (over 200) on two dimensional PAGE, at least 15 showed dramatic increases in the wounded tissue. Results indicate that wounding of tomato pericarp causes induction of synthesis and accumulation of several mRNA species while inhibiting production of relatively few mRNA species.
- Research Organization:
- Purdue Univ., West Lafayette, IN
- OSTI ID:
- 5878315
- Report Number(s):
- CONF-8707108-; TRN: 87-039114
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MESSENGER-RNA
BIOSYNTHESIS
WOUNDS
BIOLOGICAL EFFECTS
AUTORADIOGRAPHY
CHROMATOGRAPHY
ELECTROPHORESIS
ETHYLENE
GENE REGULATION
LABELLED COMPOUNDS
PLANT TISSUES
POLYPEPTIDES
SULFUR 35
TOMATOES
TRACER TECHNIQUES
ALKENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FOOD
FRUITS
HYDROCARBONS
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RADIOISOTOPES
RNA
SEPARATION PROCESSES
SULFUR ISOTOPES
SYNTHESIS
TISSUES
550201* - Biochemistry- Tracer Techniques