NMR studies of the stable mismatch purine-thymine in the self-complementary d(CGPuAATTTCG) duplex in solution
Journal Article
·
· Biochemistry; (United States)
One- and two-dimensional nuclear Overhauser effect experiments demonstrate that a single hydrogen bond between a T imino proton and purine N3 is sufficient to hold the base pair dPu-dT in d(CGPuAATTTCG) by a Watson-Crick fashion rather than a Hoogsteen type. In addition, the dPu-dT base pair is well stacked with neighboring base pairs. The spin-lattice relaxation measurements at 30 and 35/sup 0/C of two decamers, d(CGPuAATTTCG) and d(CGAAATTTCG), reveal that the elimination of two single hydrogen bonds of dA-dT base pairs (due to the substitution of adenine for purine) in the sequence results in an increase in the overall imino proton exchange rate from 7 to 36 s/sup -1/ at the site of mismatch.
- Research Organization:
- Illinois Institute of Technology, Chicago
- OSTI ID:
- 5878260
- Journal Information:
- Biochemistry; (United States), Vol. 26:18
- Country of Publication:
- United States
- Language:
- English
Similar Records
Can an Excess Electron Localise on a Purine Moiety in the Adenine-thymine Watson-Crick Base Pair? A Computational Study
NMR studies of echinomycin bisintercalation complexes with d(A1-C2-G3-T4) and d(T1-C2-G3-A4) duplexes in aqueous solution: sequence-dependent formation of Hoogsteen A1 x T4 and Watson-Crick T1 x A4 base pairs flanking the bisintercalation site
NMR and molecular modeling evidence for a Gter dot A mismatch base pair in a purine-rich DNA duplex
Journal Article
·
Tue Apr 17 00:00:00 EDT 2007
· International Journal of Quantum Chemistry, 107(12):2224-2232
·
OSTI ID:5878260
+1 more
NMR studies of echinomycin bisintercalation complexes with d(A1-C2-G3-T4) and d(T1-C2-G3-A4) duplexes in aqueous solution: sequence-dependent formation of Hoogsteen A1 x T4 and Watson-Crick T1 x A4 base pairs flanking the bisintercalation site
Journal Article
·
Tue Mar 08 00:00:00 EST 1988
· Biochemistry; (United States)
·
OSTI ID:5878260
NMR and molecular modeling evidence for a Gter dot A mismatch base pair in a purine-rich DNA duplex
Journal Article
·
Tue Jan 01 00:00:00 EST 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5878260
Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
OLIGONUCLEOTIDES
NUCLEAR MAGNETIC RESONANCE
CHEMICAL BONDS
HEAVY WATER
OVERHAUSER EFFECT
PROTONS
PURINES
SPIN-LATTICE RELAXATION
TEMPERATURE DEPENDENCE
THYMINE
AROMATICS
AZAARENES
AZINES
BARYONS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PYRIMIDINES
RELAXATION
RESONANCE
URACILS
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics
OLIGONUCLEOTIDES
NUCLEAR MAGNETIC RESONANCE
CHEMICAL BONDS
HEAVY WATER
OVERHAUSER EFFECT
PROTONS
PURINES
SPIN-LATTICE RELAXATION
TEMPERATURE DEPENDENCE
THYMINE
AROMATICS
AZAARENES
AZINES
BARYONS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PYRIMIDINES
RELAXATION
RESONANCE
URACILS
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics