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Title: Radiometric cytolysis inhibition assay, a new rapid test for neutralizing antibodies to intact and trypsin-cleaved poliovirus

We have developed a new rapid test, the radiometric cytolysis inhibition assay (RACINA), for the determination of neutralizing poliovirus antibodies. HeLa cells prelabeled with /sup 51/Cr, (/sup 3/H)leucine, or, preferentially, with (/sup 3/H)uridine are used as sensitive quantitative indicators of residual infectious virus. Both suspensions and monolayer cultures of the indicator cells can be used. Neutralization of a fraction of a high-titer virus preparation can be scored after the first replication cycle at 8 to 10 h. By lowering the incubation temperature to 30/degree/C, the completion of the cytolysis due to the first replication cycle of poliovirus was delayed beyond 21 h. This makes it possible to use the RACINA, unlike the standard microneutralization assay, for measuring antibodies to trypsin-cleaved polioviruses. The RACINA was found to be as sensitive as and more reproducible than the standard microneutralization assay in the measurement of neutralizing poliovirus antibodies. The RACINA is a rapid and reliable test for neutralizing antibodies and in principle it may be applicable for quantitation of neutralizing antibodies to other cytolytic agents as well.
Authors:
;
Publication Date:
OSTI Identifier:
5877855
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Clin. Microbiol.; (United States); Journal Volume: 27:4
Research Org:
National Public Health Institute, Helsinki (Finland)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; POLIO VIRUS; RADIOIMMUNOASSAY; VIRAL DISEASES; DIAGNOSIS; ANTIBODIES; CHROMIUM 51; HELA CELLS; LEUCINE; MAN; SENSITIVITY; SPECIFICITY; TRITIUM COMPOUNDS; URIDINE; AMINO ACIDS; ANIMALS; AZINES; BETA DECAY RADIOISOTOPES; BIOASSAY; CARBOXYLIC ACIDS; CHROMIUM ISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; MAMMALS; MICROORGANISMS; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARASITES; PRIMATES; PYRIMIDINES; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; RIBOSIDES; TRACER TECHNIQUES; URACILS; VERTEBRATES; VIRUSES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics