Growth of purified astrocytes in a chemically defined medium

Journal Article · Sun Nov 01 00:00:00 EST 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.78.11.7205· OSTI ID:5858574

Morrison, R S; De Vellis, J

Astrocytes purified from primary cultures of neonatal rat cerebrum can not be grown in a synthetic medium supplemented with putrescine, prostaglandin F/sub 2//sub ..cap alpha../, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have a marked synergistic effect on growth when used in combination but have little effect when used individually. Astrocytes grown in the defined medium exhibit dramatic changes in morphological characteristics in comparison to cells grown in serum-free or serum-supplemented medium. In addition, these cells express the astrocyte-specific marker glial fibrillary acidic protein and are estimated by several criteria to be greater than 95% astrocytes.

• Research Organization: Univ. of California, Los Angeles, CA (United States)
• DOE Contract Number: AM03-76SF00012
• OSTI ID: 5858574
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 78:11
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
BIOLOGICAL EFFECTS
HYDROCORTISONE
INSULIN
NERVE CELLS
GROWTH
PROSTAGLANDINS
PUTRESCINE
CEREBRUM
MORPHOLOGICAL CHANGES
NEONATES
RATS
SYNERGISM
ADRENAL HORMONES
AMINES
ANIMAL CELLS
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PREGNANES
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
VERTEBRATES
550300* - Cytology