DNA hybridization assay for detection of gypsy moth nuclear polyhedrosis virus in infected gypsy moth (Lymantria dispar L. ) larvae

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:5858291

Keating, S T; Burand, J P; Elkinton, J S ^[1]

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1. Univ. of Massachusetts, Amherst (USA)

Radiolabeled Lymantria dispar nuclear polyhedrosis virus DNA probes were used in a DNA hybridization assay to detect the presence of viral DNA in extracts from infected larvae. Total DNA was extracted from larvae, bound to nitrocellulose filters, and assayed for the presence of viral DNA by two methods: slot-blot vacuum filtration and whole-larval squashes. The hybridization results were closely correlated with mortality observed in reared larvae. Hybridization of squashes of larvae frozen 4 days after receiving the above virus treatments also produced accurate measures of the incidence of virus infection.

• OSTI ID: 5858291
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 55:11; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LARVAE
MORTALITY
RECOMBINANT DNA
BIOASSAY
VIRUSES
INFECTIVITY
DNA HYBRIDIZATION
LYMANTRIA DISPAR
ANIMALS
ARTHROPODS
DNA
HYBRIDIZATION
INSECTS
INVERTEBRATES
LEPIDOPTERA
MICROORGANISMS
MOTHS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
550201* - Biochemistry- Tracer Techniques