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Title: Uptake and release of adrenal ascorbic acid in the guinea pig after injection of ACTH

Abstract

The effect of a single injection of ACTH (3 IU/100 g body weight) on the distribution of ascorbic acid (AA) and radiolabeled AA in 20 tissues was studied in adult male guinea pigs consuming 500 mg AA/kg diet. Saline- or ACTH-injected animals were simultaneously injected with (1-/sup 14/C)AA, and killed at 0.5, 1, 2, 4 and 6 h after injection. There was no significant difference between treatments in the weight of any tissue over the 6-h experimental period. As anticipated, the concentration of AA in the adrenals of animals injected with ACTH was 33% of that of animals injected with saline at 4 h. Unexpectedly, the concentration of radiolabeled AA in the adrenals at 0.5 h after ACTH injection was 172% of that after saline injection. The concentration of radiolabeled AA in the adrenal of the saline-injected animals increased slowly over time to reach a level similar to that of ACTH-injected animals by 6 h. There was no effect of ACTH on the level of AA or uptake in any of the other tissues examined. These results demonstrate that a single dose of ACTH markedly influences the retention of AA in the adrenal gland without similarly altering retention of AAmore » in other tissues. Furthermore, ACTH treatment causes both accelerated uptake and release of AA into the adrenals.« less

Authors:
;
Publication Date:
Research Org.:
Cornell Univ., Ithaca, NY
OSTI Identifier:
5856909
Resource Type:
Journal Article
Journal Name:
J. Nutr.; (United States)
Additional Journal Information:
Journal Volume: 117:9
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ASCORBIC ACID; TISSUE DISTRIBUTION; HYDROCORTISONE; BIOLOGICAL FUNCTIONS; ADRENAL GLANDS; CARBON 14 COMPOUNDS; GUINEA PIGS; ADRENAL HORMONES; ANIMALS; BODY; CORTICOSTEROIDS; DISTRIBUTION; ENDOCRINE GLANDS; FUNCTIONS; GLANDS; GLUCOCORTICOIDS; HYDROXY COMPOUNDS; KETONES; LABELLED COMPOUNDS; MAMMALS; ORGANIC COMPOUNDS; ORGANS; PREGNANES; RODENTS; STEROIDS; VERTEBRATES; VITAMINS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Kipp, D E, and Rivers, J M. Uptake and release of adrenal ascorbic acid in the guinea pig after injection of ACTH. United States: N. p., 1987. Web.
Kipp, D E, & Rivers, J M. Uptake and release of adrenal ascorbic acid in the guinea pig after injection of ACTH. United States.
Kipp, D E, and Rivers, J M. 1987. "Uptake and release of adrenal ascorbic acid in the guinea pig after injection of ACTH". United States.
@article{osti_5856909,
title = {Uptake and release of adrenal ascorbic acid in the guinea pig after injection of ACTH},
author = {Kipp, D E and Rivers, J M},
abstractNote = {The effect of a single injection of ACTH (3 IU/100 g body weight) on the distribution of ascorbic acid (AA) and radiolabeled AA in 20 tissues was studied in adult male guinea pigs consuming 500 mg AA/kg diet. Saline- or ACTH-injected animals were simultaneously injected with (1-/sup 14/C)AA, and killed at 0.5, 1, 2, 4 and 6 h after injection. There was no significant difference between treatments in the weight of any tissue over the 6-h experimental period. As anticipated, the concentration of AA in the adrenals of animals injected with ACTH was 33% of that of animals injected with saline at 4 h. Unexpectedly, the concentration of radiolabeled AA in the adrenals at 0.5 h after ACTH injection was 172% of that after saline injection. The concentration of radiolabeled AA in the adrenal of the saline-injected animals increased slowly over time to reach a level similar to that of ACTH-injected animals by 6 h. There was no effect of ACTH on the level of AA or uptake in any of the other tissues examined. These results demonstrate that a single dose of ACTH markedly influences the retention of AA in the adrenal gland without similarly altering retention of AA in other tissues. Furthermore, ACTH treatment causes both accelerated uptake and release of AA into the adrenals.},
doi = {},
url = {https://www.osti.gov/biblio/5856909}, journal = {J. Nutr.; (United States)},
number = ,
volume = 117:9,
place = {United States},
year = {Tue Sep 01 00:00:00 EDT 1987},
month = {Tue Sep 01 00:00:00 EDT 1987}
}