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Title: Ribulose diphosphate carboxylase of the cyanobacterium Spirulina platensis

The ribulose diphosphate (RDP) carboxylase activity of the cyanobacterium Spirulina platensis is represented by two peaks when a cell homogenate is centrifuged in a sucrose density gradient. In the case of differential centrifugation (40,000 g, 1 h), the activity of the enzyme was distributed between the supernatant liquid (soluble form) and the precipitate (carboxysomal form). From the soluble fraction, in which 80-95% of the total activity of the enzyme is concentrated, electrophoretically homogeneous RDP carboxylase was isolated by precipitation with ammonium sulfate and centrifugation in a sucrose density gradient. The purified enzyme possessed greater electrophoretic mobility in comparison with the RDP carboxylase of beans Vicia faba. The molecular weight of the enzyme, determined by gel filtration, was 450,000. The enzyme consists of monotypic subunits with a molecular weight of 53,000. The small subunits were not detected in electrophoresis in polyacrylamide gel in the presence of SDS after fixation and staining of the gels by various methods.
Authors:
; ;
Publication Date:
OSTI Identifier:
5856545
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemistry (Engl. Transl.); (United States); Journal Volume: 51:5; Other Information: Translated from Biokhimiya; 51: No. 5, 816-822(May 1986)
Research Org:
A.N. Bakh Institute of Biochemistry, Moscow, USSR
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBOXY-LYASES; ELECTROPHORESIS; ENZYME ACTIVITY; RADIOENZYMATIC ASSAY; CYANOBACTERIA; CARBON 14 COMPOUNDS; DIALYSIS; METABOLISM; MOLECULAR WEIGHT; ORGANIC PHOSPHORUS COMPOUNDS; RIBULOSE; UPTAKE; CARBOHYDRATES; CARBON-CARBON LYASES; ENZYMES; KETONES; LABELLED COMPOUNDS; LYASES; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PENTOSES; SACCHARIDES; SEPARATION PROCESSES 550201* -- Biochemistry-- Tracer Techniques