Ribulose diphosphate carboxylase of the cyanobacterium Spirulina platensis
The ribulose diphosphate (RDP) carboxylase activity of the cyanobacterium Spirulina platensis is represented by two peaks when a cell homogenate is centrifuged in a sucrose density gradient. In the case of differential centrifugation (40,000 g, 1 h), the activity of the enzyme was distributed between the supernatant liquid (soluble form) and the precipitate (carboxysomal form). From the soluble fraction, in which 80-95% of the total activity of the enzyme is concentrated, electrophoretically homogeneous RDP carboxylase was isolated by precipitation with ammonium sulfate and centrifugation in a sucrose density gradient. The purified enzyme possessed greater electrophoretic mobility in comparison with the RDP carboxylase of beans Vicia faba. The molecular weight of the enzyme, determined by gel filtration, was 450,000. The enzyme consists of monotypic subunits with a molecular weight of 53,000. The small subunits were not detected in electrophoresis in polyacrylamide gel in the presence of SDS after fixation and staining of the gels by various methods.
- Research Organization:
- A.N. Bakh Institute of Biochemistry, Moscow, USSR
- OSTI ID:
- 5856545
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 51:5; Other Information: Translated from Biokhimiya; 51: No. 5, 816-822(May 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXY-LYASES
ELECTROPHORESIS
ENZYME ACTIVITY
RADIOENZYMATIC ASSAY
CYANOBACTERIA
CARBON 14 COMPOUNDS
DIALYSIS
METABOLISM
MOLECULAR WEIGHT
ORGANIC PHOSPHORUS COMPOUNDS
RIBULOSE
UPTAKE
CARBOHYDRATES
CARBON-CARBON LYASES
ENZYMES
KETONES
LABELLED COMPOUNDS
LYASES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
SACCHARIDES
SEPARATION PROCESSES
550201* - Biochemistry- Tracer Techniques