Measurement of urinary histamine: comparison of fluorometric and radioisotopic-enzymatic assay procedures

Journal Article · Mon Feb 01 00:00:00 EST 1982 · J. Allergy Clin. Immunol.; (United States)

OSTI ID:5855917

Warren, K; Dyer, J; Merlin, S; Kaliner, M

Assessment of urinary histamine may prove useful in determining the role of histamine in human health and disease. Urinary histamine may be accurately estimated by a modified fluorometric assay employing diamine oxidase (DAO) digestion and cation-exchange chromatography. However, the radioisotopic-enzymatic assay is less expensive, easier to perform, and possibly more sensitive. Therefore the two procedures were compared. The radioenzyme assay was found to be affected by factors in urine (possibly salt concentrations) requiring extraction of histamine from urine by butanol-heptane. Moreover, it was found to be necessary to compare DAO-digested samples with undigested samples to accurately estimate histamine levels and to run the standard curve of histamine in DAO-digested urine. Even with these modifications, the radioenzyme assay was not as accurate as the fluorometric assay for urine samples having histamine values about 60 ng/ml. Therefore we recommend utilization of the modified fluorometric assay for the measurement of urinary histamine levels.

• Research Organization: National Inst. of Health, Bethesda, MD
• OSTI ID: 5855917
• Journal Information: J. Allergy Clin. Immunol.; (United States), Vol. 71:2
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
HISTAMINE
FLUORESCENCE SPECTROSCOPY
RADIOASSAY
RADIOISOTOPES
HISTAMINASE
MAN
URINE
AMINE OXIDASES
AMINES
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
EMISSION SPECTROSCOPY
ENZYMES
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PRIMATES
SPECTROSCOPY
VERTEBRATES
WASTES
550601* - Medicine- Unsealed Radionuclides in Diagnostics