An enzymic radiochemical method for determining phosphatidylglycerol in amniotic fluid

Journal Article · Sun May 01 00:00:00 EDT 1983 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5855369

Siegel, L; Walker, S I; Robin, N I

We describe an enzymic quantification of phosphatidylglycerol in amniotic fluid. Phosphatidylglycerol is hydrolyzed in alkali and the resulting glycerol is then enzymatically phosphorylated with adenosine 5'-(gamma-/sup 32/P)triphosphate to yield glycero(/sup 32/P)phosphate. After removal of excess (gamma-/sup 32/P)ATP by charcoal, the radioactivity of the glycerophosphate is measured in a liquid scintillation counter. Triglyceride in the amniotic fluid is hydrolyzed by lipase before extraction and thus does not interfere with the analysis. This method is specific for phosphatidylglycerol. Preliminary studies suggest that a phosphatidylglycerol value greater than or equal to 10 nmol/mL correlates with fetal lung maturity.

• Research Organization: Department of Laboratories, Stamford Hospital, CT
• OSTI ID: 5855369
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 29:5
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
PHOSPHOLIPIDS
RADIOENZYMATIC ASSAY
AMNIOTIC FLUID
GLYCEROL
PHOSPHORUS 32
TRIGLYCERIDES
ALCOHOLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ESTERS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics