Improved determination of manganese in hair by use of a mini-autoclave and flameless atomic absorption spectrometry with Zeeman background correction: an evaluation in unexposed subjects

Journal Article · Mon Oct 01 00:00:00 EDT 1984 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5852742

Guillard, O; Brugier, J C; Piriou, A; Menard, M; Gombert, J; Reiss, D

Motivated by the relatively wide range of values published hitherto, we offer a new, reliable method for determination of manganese in hair from unexposed human subjects. By using a mini-autoclave, we have developed a method that obviates loss of manganese during digestion and, thanks to use of a Teflon receptacle and rigorous methodology, contamination has been minimized. We used Zeeman flameless atomic absorption spectrometry, with the method of standard additions, for quantification. Within-run CVs for concentrations of Mn of 0.26 and 0.28 microgram/g of dry hair were 3.63 and 3.93%; the day-to-day CV for a Mn concentration of 0.29 microgram/g of dry hair was 5.84%. Mean (+/- SD) analytical recovery of Mn added to samples of hair was 104% (+/- 9.2%). The mean concentration of Mn found in the hair of 15 healthy unexposed subjects was 0.26 (+/- 0.05) microgram/g dry weight. This sensitive, reproducible procedure is suitable for use in analysis for traces of Mn in hair.

• Research Organization: Jean Bernard Hospital, Poitiers, France
• OSTI ID: 5852742
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 30:10
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HAIR
CHEMICAL ANALYSIS
MANGANESE COMPOUNDS
ABSORPTION SPECTROSCOPY
AUTOCLAVES
ZEEMAN EFFECT
BODY
ORGANS
SKIN
SPECTROSCOPY
TRANSITION ELEMENT COMPOUNDS
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)