Isolation of cell cycle-dependent gamma ray-sensitive Chinese hamster ovary cell
A technique for the isolation of gamma ray-sensitive Chinese hamster ovary (CHO) cell mutants is described, which uses nylon cloth replica plating and photography with dark-field illumination to directly monitor colonies for growth after gamma irradiation. Two gamma ray-sensitive mutants were isolated using this method. One of these cells (XR-1) had a two-slope survival curve: an initial steep slope and then a flattening of the curve at about 10% survival. Subsequently, it was found that this cell is sensitive to gamma irradiation in G1, early S, and late G2 phases of the cell cycle, whereas in the resistant phase (late S phase) its survival approaches that of the parental cells. The D37 in the sensitive G1 period is approximately 30 rads, compared with 300 rads of the parental cell. This mutant cell is also sensitive to killing by the DNA breaking agent, bleomycin, but is relatively insensitive to UV light and ethyl methane sulfonate, suggesting that the defect is specific for agents that produce DNA strand breakage.
- Research Organization:
- Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania
- OSTI ID:
- 5849707
- Journal Information:
- Somatic Cell Genet.; (United States), Vol. 9:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Radiosensitivity, apoptosis and repair of DNA double-strand breaks in radiation-sensitive Chinese hamster ovary cell mutants treated at different dose rates
The use of DNA-repair-deficient mutants of Chinese hamster ovary cells in studying mutagenesis mechanisms and testing for environmental mutagens
Related Subjects
CHO CELLS
MUTANTS
RADIOSENSITIVITY
BLEOMYCIN
CELL CYCLE
DNA
EMS
GAMMA RADIATION
SEPARATION PROCESSES
STRAND BREAKS
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
IONIZING RADIATIONS
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
SULFONIC ACID ESTERS
560121* - Radiation Effects on Cells- External Source- (-1987)