The metabolism of polycyclic aromatic hydrocarbons by two marine fishes: the little skate, Raja erinacea and the winter flounder, Pseudopleuronectes americanus
Hepatic cytosolic fractions from male little skates exhibited high specific rates of enzymatic conjugation of glutathione with the polycyclic aromatic hydrocarbon (PAH) oxide, benzo(a)pyrene 4,5-oxide (4,5-BPO). High performance liquid chromatography analysis demonstrated that all hepatic skate tranferases showed strict configurational stereoselectivity for the R carbons in enantiomeric 4,5-BPO, only the S products were observed in enzymic reaction mixtures. Rates of metabolism of the PAH benzo(a)pyrene (i.e., AHH activity) varied markedly in hepatic homogenates prepared from feral winter flounder caught in the Gulf of Maine. Treatment of feral flounder with either a PAH or a PAH-type compound resulted in induced AHH activities in the liver of all flounder so treated. ANF (7,8-benzoflavone) acted to inhibit the hepatic AHH activity in all treated fish as it did in 64% of the feral flounder examined. Electrophoretic analysis of hepatic microsomes from flounder treated with either a PAH or a PAH-type compound showed a novel or enriched polypeptide species at approximately 57,000 MW. A polypeptide species of similar MW was only faintly discernable in hepatic microsomes from feral flounder whose hepatic AHH activities were much lower than that of the treated flounder whereas a band of similar molecular weight was prominent in hepatic microsomes from feral flounder with high hepatic AHH activities. These results suggest that many of the flounder in this area of Maine have induction of their hepatic monooxygenase systems similar to that caused by PAH or PAH-type compounds.
- OSTI ID:
- 5848021
- Resource Relation:
- Other Information: Thesis (Ph.D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
POLYCYCLIC AROMATIC HYDROCARBONS
METABOLISM
BENZOPYRENE
FISHES
GLUTATHIONE
LIVER
METABOLIC ACTIVATION
MICROSOMES
TRANSFERASES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BODY
CELL CONSTITUENTS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DRUGS
ENZYMES
GLANDS
HYDROCARBONS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)