Highly sensitive and reproducible immunoradiometric assay for total human renin using monoclonal antibodies, iodogen labelling and polystyrene star tubes
A two-site immunoradiometric assay for total renin concentration in human plasma is described. A new type of polystyrene tubes with a special bottom geometry, so-called Star Tubes were used. The lower limit of detection was 2 microIU per ml and the working range from 2 to 2000 microIU per ml. The variation for duplicate determinations on standards and plasma samples was 2.7% and the day to day variation was 4.8%. No significant interferences or cross reactivities were identified. EDTA-plasma was analyzed after dilution with a phosphate buffer. Plasma samples from different patient categories were analyzed. The results were compared with those obtained by our substrate enrichment method after acid activation of the samples had taken place. A linear correlation (r = 0.990) between the results obtained with the two methods was found.
- Research Organization:
- Univ. of Copenhagen, Denmark
- OSTI ID:
- 5842004
- Journal Information:
- Clin. Exp. Hypertens., Part A; (United States), Vol. 9:8-9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIABETES MELLITUS
PATHOGENESIS
HYPERTENSION
RENIN
RADIOIMMUNOASSAY
MAN
MONOCLONAL ANTIBODIES
ANIMALS
ANTIBODIES
CARDIOVASCULAR DISEASES
DISEASES
ENDOCRINE DISEASES
ENZYMES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
METABOLIC DISEASES
NONSPECIFIC PEPTIDASES
PEPTIDE HYDROLASES
PRIMATES
RADIOASSAY
RADIOIMMUNOLOGY
SYMPTOMS
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques