Partial characterization of rhamnogalacturonan I from suspension-cultured cotton cell walls
- Oklahoma State Univ., Stillwater (USA)
It is most likely that the backbone of rhamnogalacturonan I (RGI) in cotton is a repeating disaccharide of rhamnose and galacturonic acid, frequently 3-O-acetylated. After endopolygalacturonase treatment of cotton walls, polymeric RGI can be solubilized by strong alkali extraction or cellulase digestion. All of the RGI along with xyloglucan is solubilized by the alkali, whereas only {approximately}50% is solubilized by the cellulase which digests the xyloglucan. The polymeric material solubilized by cellulase was shown by {sup 13}C and {sup 1}H NMR spectroscopy to be quite highly acetylated and to have some of its galacturonic acid residues methylesterified. From compositional analysis, and assuming a repeating (galA-rha) n backbone for RGI proper, it appears that a considerable amount of homogalacturonan containing {approximately}20% methylesterified galA co-solubilized with the RGI and is possibly covalently attached to it. Progress towards the structural characterization of the RGI and its associated polymers will be presented.
- OSTI ID:
- 5841758
- Report Number(s):
- CONF-9007196-; CODEN: PPYSA
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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