Polysaccharide fraction from higher plants which strongly interacts with the cytosolic phosphorylase isozyme. I. Isolation and characterization. [Spinacia oleracea L. ; Pisum sativum L]

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.3.960· OSTI ID:5841656

Yang, Yi; Steup, M ^[1]

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1. Botanisches Institut der Westfaelischen Wilhelms-Universitaet Muenster (West Germany)

From leaves of Spinacia oleracea L. or from Pisum sativum L. and from cotyledons of germinating pea seeds a high molecular weight polysaccharide fraction was isolated. The apparent size of the fraction, as determined by gel filtration, was similar to that of dextran blue. Following acid hydrolysis the monomer content of the polysaccharide preparation was studied using high pressure liquid and thin layer chromatography. Glucose, galactose, arabinose, and ribose were the main monosaccharide compounds. The native polysaccharide preparation interacted strongly with the cytosolic isozyme of phosphorylase (EC 2.4.1.1). Interaction with the plastidic phosphorylase isozyme(s) was by far weaker. Interaction with the cytosolic isozyme was demonstrated by affinity electrophoresis, kinetic measurements, and by {sup 14}C-labeling experiments in which the glucosyl transfer from ({sup 14}C)glucose 1-phosphate to the polysaccharide preparation was monitored.

• OSTI ID: 5841656
• Journal Information: Plant Physiology; (USA), Vol. 94:3; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOSYL TRANSFERASES
BIOCHEMICAL REACTION KINETICS
PISUM
PHYSIOLOGY
POLYSACCHARIDES
SPINACH
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
CARBOHYDRATES
ENZYMES
FOOD
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PLANTS
REACTION KINETICS
SACCHARIDES
TRANSFERASES
VEGETABLES
550201* - Biochemistry- Tracer Techniques