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Title: Induction of mutagenesis and transformation in BALB/c-3T3 clone A31-1 cells by diverse chemical carcinogens

BALB/c-3T3 cells were employed to examine the genotoxic potential of a variety of known chemical carcinogens. BALB/c-3T3 cells displayed a dose-dependent transformation response to a variety of carcinogens (polycyclic hydrocarbons, methylating agents, ethylating agents, aflatoxin B{sub 1} (AFT{sub 1}), and 4-nitroquinoline-N-oxide (4-NQO)). When the ability of these compounds to induce mutagenesis to resistance to the cardiac glycoside ouabain (OUA{sup R}) was examined, the authors found the short chain alkylating agents to be particularly effective mutagens, causing biologic effects at doses below those necessary to induce a transformation response. In contrast, the polycyclic hydrocarbons which were potent transforming agents were weaker, albeit significant, mutagens for the OUA{sup R} locus in this system, while AFB{sub 1} was quite weak. Further studies were performed with 5-azacytidine (5-AZA) and the nongenotoxic carcinogen cinnamyl anthranilate (CIN). 5-AZA was a potent transforming agent, but failed to cause mutagenesis. CIN similarly caused in vitro transformation. When a series of eight structurally diverse compounds were examined in both the BALB/c-3T3 and C3H10T1/2 mouse fibroblast transformation systems, the BALB/c-3T3 system was shown to be sensitive to a wide variety of potential carcinogens, whereas the C3H10T1/2 system proved routinely sensitive only to the polycyclic hydrocarbons.
Authors:
 [1] ; ; ; ;  [2]
  1. (National Cancer Institute, Frederick, MD (USA))
  2. (Microbiological Associates, Bethesda, MD (USA))
Publication Date:
OSTI Identifier:
5840058
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental and Molecular Mutagenesis; (USA); Journal Volume: 16:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CARCINOGENS; GENETIC EFFECTS; FIBROBLASTS; ONCOGENIC TRANSFORMATIONS; AFLATOXINS; BENZOPYRENE; MUTAGENESIS; NITROSO COMPOUNDS; POLYCYCLIC AROMATIC HYDROCARBONS; POLYCYCLIC NITRO COMPOUNDS; QUINOLINES; ANIMAL CELLS; ANTIGENS; AROMATICS; AZAARENES; AZINES; BIOLOGICAL EFFECTS; CELL TRANSFORMATIONS; CONDENSED AROMATICS; CONNECTIVE TISSUE CELLS; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; MATERIALS; NITRO COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIDINES; SOMATIC CELLS; TOXIC MATERIALS; TOXINS 560300* -- Chemicals Metabolism & Toxicology