Characterization of an inducible UDP-glucose:salicylic acid O-glucosyltransferase from oat roots
- Univ. of Wisconsin, Madison (USA)
Phytotoxicity of salicylic acid (SA), a phenolic acid that inhibits ion absorption in plant roots, is reduced in oat roots by the action of a UDP-glucose:SA glucosyltransferase (GTase). GTase activity, extracted from oat roots and assayed with ({sup 14}C)SA, was present at low constitutive levels but increased within 1.5 h of incubation of roots in 0.5 mM SA at pH 6.5. This induction was the result of de novo RNA and protein synthesis. Induction was highly specific towards SA as the inducer. The partially purified, soluble enzyme has a M{sub t} of about 50,000 and high specificity towards UDP-glucose as the sugar donor (K{sub m} = 0.28 mM) and SA as the glucose acceptor (K{sub m} = 0.11 mM). 2-D PAGE of ({sup 35}S)methionine-labeled proteins extracted from induced and uninduced roots revealed a candidate peptide representing the GTase. This peptide was also present on gels of partially purified GTase.
- OSTI ID:
- 5839638
- Report Number(s):
- CONF-9007196-; CODEN: PPYSA
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
SALICYLIC ACID
BIOLOGICAL EFFECTS
TOXICITY
GLYCOSYL TRANSFERASES
METHIONINE
OATS
SULFUR 35
SULFUR COMPLEXES
SULFUR COMPOUNDS
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CEREALS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
GRAMINEAE
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LILIOPSIDA
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIOISOTOPES
SULFUR ISOTOPES
TRANSFERASES
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques