Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5832386

Datta, A R; Wentz, B A; Hill, W E

A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.

• Research Organization: Food and Drug Administration, Washington, DC
• OSTI ID: 5832386
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 53:9
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
HYBRIDIZATION
MICROWAVE RADIATION
GENETIC EFFECTS
BACTERIA
GENES
GENETIC VARIABILITY
HEMOLYSINS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
ELECTROMAGNETIC RADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
560400* - Other Environmental Pollutant Effects