Xenobiotics in gametes of Lake Michigan lake trout (Salvelinus namaycush) induce hepatic monooxygenase activity in their offspring
Eggs spawned from Lake Michigan lake trout contain a number of xenobiotic compounds, including polychlorinated biphenyls (PCBs). To assess whether this contamination is sufficient to induce hepatic cytochrome P-450-dependent monooxygenase (MO) activity during early development, the hepatic MO systems of laboratory-cultured offspring of Lake Michigan, Green Bay, and Marquette Hatchery lake trout were compared. Additionally, the induction of hepatic cytochrome P-450 systems in developing lake trout by the commercial PCB mixture, Aroclor 1254 (A1254), was characterized. During late embryonic development and at the swim-up stage, the hepatic MO systems of the feral lake trout offspring appeared induced, based on levels of aryl hydrocarbon hydroxylase (AHH) activity that were 3.5- to 8.6-fold higher than the hatchery control levels. Furthermore, at the swim-up stage the feral trout offspring resembled A1254-treated hatchery fry with regard to the degree of inhibition of hepatic AHH activity by alpha-naphthoflavone, and the presence of an inducible Mr . 58,000 polypeptide in hepatic microsomes. The levels of aminopyrine N-demethylase activity, which was relatively unresponsive to inducers, were moderately lower in the Lake Michigan and Green Bay swim-up fry compared to the hatchery control levels. After 7 months of posthatching laboratory culture, when residues of xenobiotics present at fertilization were greatly diluted by growth, the hepatic MO systems of the Lake Michigan and hatchery trout offspring appeared essentially indistinguishable with regard to a number of parameters.
- Research Organization:
- Medical Coll. of Wisconsin, Milwaukee
- OSTI ID:
- 5830113
- Journal Information:
- Fundam. Appl. Toxicol.; (United States), Vol. 4:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHLORINATED AROMATIC HYDROCARBONS
TOXICITY
LAKE MICHIGAN
WATER POLLUTION
OXYGENASES
ENZYME ACTIVITY
TROUT
CONTAMINATION
COMPARATIVE EVALUATIONS
CYTOCHROMES
EGGS
FISHERIES
LIVER
MICROSOMES
XENOBIOTICS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENZYMES
FISHES
GLANDS
GREAT LAKES
HALOGENATED AROMATIC HYDROCARBONS
LAKES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PIGMENTS
POLLUTION
SURFACE WATERS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)