Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage

Tyrrell, R M

doi:10.1073/pnas.81.3.781

Title: Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage

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Abstract

Nondividing (arrested) populations of primary human fibroblasts from normal individuals exposed to an intial dose (1.5 or 3 Jm/sup -2/) of far-UV (254 nm) radiation and then incubated in medium containing low (0.5%) serum develop enhanced resistance to inactivation of cloning efficiency by a second (challenge) dose of UV. The resistance develops within 2-4 days, after which there is a decline. Resistance develops to a higher degree and more rapidly (1-2 days) in cells derived from patients with the variant form of xeroderma pigmentosum. Excision-deficient cells from xeroderma pigmentosum complementation group A individuals also develop UV resistance after a lower (0.2 Jm/sup -2/) exposure to UV. Enhanced UV resistance does not develop in UV-irradiated cell populations incubated with the protein synthesis inhibitor cycloheximide (5 ..mu..M). These observations are consistent with the interpretation that exposure of human fibroblasts to low doses of UV induces synthesis of a protein involved in a metabolic pathway that transiently enhances the capacity of cells to repair potentially lethal damage resulting from a subsequent dose of UV.

Authors:: Tyrrell, R M

Publication Date:: Wed Feb 01 00:00:00 EST 1984

Research Org.:: Swiss Institute for Experimental Cancer Research, Lausanne

OSTI Identifier:: 5825974

Resource Type:: Journal Article

Journal Name:: Proc. Natl. Acad. Sci. U.S.A.; (United States)

Additional Journal Information:: Journal Volume: 81:3

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BIOLOGICAL REPAIR; STIMULATION; FIBROBLASTS; LOW DOSE IRRADIATION; RADIOSENSITIVITY; BIOLOGICAL PATHWAYS; CLONING; CYCLOHEXIMIDE; EXPERIMENTAL DATA; FAR ULTRAVIOLET RADIATION; PATIENTS; XERODERMA PIGMENTOSUM; ANIMAL CELLS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; BIOLOGICAL RECOVERY; CONNECTIVE TISSUE CELLS; DATA; DISEASES; DRUGS; ELECTROMAGNETIC RADIATION; FUNGICIDES; INFORMATION; IRRADIATION; NUMERICAL DATA; PESTICIDES; RADIATIONS; RECOVERY; REPAIR; SKIN DISEASES; SOMATIC CELLS; ULTRAVIOLET RADIATION; 560121* - Radiation Effects on Cells- External Source- (-1987)

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                    Tyrrell, R M. Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage.  United States: N. p., 1984. 
        Web.  doi:10.1073/pnas.81.3.781.

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                    Tyrrell, R M. Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage.  United States.  https://doi.org/10.1073/pnas.81.3.781

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                    Tyrrell, R M. 1984.  
        "Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage".  United States.  https://doi.org/10.1073/pnas.81.3.781.

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@article{osti_5825974,

  title        = {Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage},

  author       = {Tyrrell, R M},

  abstractNote = {Nondividing (arrested) populations of primary human fibroblasts from normal individuals exposed to an intial dose (1.5 or 3 Jm/sup -2/) of far-UV (254 nm) radiation and then incubated in medium containing low (0.5%) serum develop enhanced resistance to inactivation of cloning efficiency by a second (challenge) dose of UV. The resistance develops within 2-4 days, after which there is a decline. Resistance develops to a higher degree and more rapidly (1-2 days) in cells derived from patients with the variant form of xeroderma pigmentosum. Excision-deficient cells from xeroderma pigmentosum complementation group A individuals also develop UV resistance after a lower (0.2 Jm/sup -2/) exposure to UV. Enhanced UV resistance does not develop in UV-irradiated cell populations incubated with the protein synthesis inhibitor cycloheximide (5 ..mu..M). These observations are consistent with the interpretation that exposure of human fibroblasts to low doses of UV induces synthesis of a protein involved in a metabolic pathway that transiently enhances the capacity of cells to repair potentially lethal damage resulting from a subsequent dose of UV.},

  doi          = {10.1073/pnas.81.3.781},

  url          = {https://www.osti.gov/biblio/5825974},
  journal      = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number       = ,

  volume       = 81:3,

  place        = {United States},

  year         = {Wed Feb 01 00:00:00 EST 1984},

  month        = {Wed Feb 01 00:00:00 EST 1984}

}

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