Photobinding of /sup 3/H 8-methoxypsoralen to monkey intraocular tissues

Lerman, S; Megaw, J; Gardner, K; Takei, Y; Franks, Y; Gammon, A

Title: Photobinding of /sup 3/H 8-methoxypsoralen to monkey intraocular tissues

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Abstract

Young (less than 1 year) and old (greater than 15 years) Rhesus monkeys were utilized in this study in order to determine whether ultraviolet (UV) radiation at ambient levels induces psoralen photobinding in primate eyes (in particular the lens and retina). Unilateral aphakia or pseudophakia was induced surgically and the eyes were allowed to heal. The animals then were given a single intraperitoneal injection of /sup 3/H 8-methoxypsoralen (8-MOP) and immediately exposed to BLB lights (of measured radiation intensity at the corneal surface). The animals were killed at varying time periods (2-6 weeks), and the eyes were removed immediately. One-half of each cornea and lens was frozen for subsequent optical spectroscopy and the remaining ocular tissues were fixed for histopathologic studies and autoradiography. These data demonstrate that low level UV radiation (less than 0.4 mW/cm2) can cause 8-MOP photobinding to lens proteins and DNA and to aphakic, pseudophakic, and young phakic primate retinas. The older phakic primate lens serves as a protective UV filter and prevents psoralen photobinding within the retina. These data suggest that older aphakes and pseudophakes may require UV radiation protection to prevent direct as well as photosensitized retinal photodamage.

Authors:: Lerman, S; Megaw, J; Gardner, K; Takei, Y; Franks, Y; Gammon, A

Publication Date:: Thu Nov 01 00:00:00 EST 1984

Research Org.:: Emory Univ., Atlanta, GA

OSTI Identifier:: 5824198

Resource Type:: Journal Article

Journal Name:: Invest. Ophthalmol. Visual Sci.; (United States)

Additional Journal Information:: Journal Volume: 25:11

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; EYES; INJURIES; PSORALEN; TOXICITY; ULTRAVIOLET RADIATION; AUTORADIOGRAPHY; INTRAPERITONEAL INJECTION; MONKEYS; TRITIUM COMPOUNDS; ANIMALS; ANTICOAGULANTS; BODY; BODY AREAS; COUMARINS; DRUGS; ELECTROMAGNETIC RADIATION; FACE; HEAD; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; INJECTION; INTAKE; LABELLED COMPOUNDS; MAMMALS; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANS; PRIMATES; RADIATIONS; SENSE ORGANS; VERTEBRATES; 560400* - Other Environmental Pollutant Effects; 550901 - Pathology- Tracer Techniques

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                    Lerman, S, Megaw, J, Gardner, K, Takei, Y, Franks, Y, and Gammon, A. Photobinding of /sup 3/H 8-methoxypsoralen to monkey intraocular tissues.  United States: N. p., 1984. 
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                    Lerman, S, Megaw, J, Gardner, K, Takei, Y, Franks, Y, & Gammon, A. Photobinding of /sup 3/H 8-methoxypsoralen to monkey intraocular tissues.  United States.

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                    Lerman, S, Megaw, J, Gardner, K, Takei, Y, Franks, Y, and Gammon, A. 1984.  
        "Photobinding of /sup 3/H 8-methoxypsoralen to monkey intraocular tissues".  United States.

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@article{osti_5824198,

  title        = {Photobinding of /sup 3/H 8-methoxypsoralen to monkey intraocular tissues},

  author       = {Lerman, S and Megaw, J and Gardner, K and Takei, Y and Franks, Y and Gammon, A},

  abstractNote = {Young (less than 1 year) and old (greater than 15 years) Rhesus monkeys were utilized in this study in order to determine whether ultraviolet (UV) radiation at ambient levels induces psoralen photobinding in primate eyes (in particular the lens and retina). Unilateral aphakia or pseudophakia was induced surgically and the eyes were allowed to heal. The animals then were given a single intraperitoneal injection of /sup 3/H 8-methoxypsoralen (8-MOP) and immediately exposed to BLB lights (of measured radiation intensity at the corneal surface). The animals were killed at varying time periods (2-6 weeks), and the eyes were removed immediately. One-half of each cornea and lens was frozen for subsequent optical spectroscopy and the remaining ocular tissues were fixed for histopathologic studies and autoradiography. These data demonstrate that low level UV radiation (less than 0.4 mW/cm2) can cause 8-MOP photobinding to lens proteins and DNA and to aphakic, pseudophakic, and young phakic primate retinas. The older phakic primate lens serves as a protective UV filter and prevents psoralen photobinding within the retina. These data suggest that older aphakes and pseudophakes may require UV radiation protection to prevent direct as well as photosensitized retinal photodamage.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5824198},
  journal      = {Invest. Ophthalmol. Visual Sci.; (United States)},
number       = ,

  volume       = 25:11,

  place        = {United States},

  year         = {Thu Nov 01 00:00:00 EST 1984},

  month        = {Thu Nov 01 00:00:00 EST 1984}

}

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