Structure and expression of mouse VL30 genes
DNA sequencing and blot hybridization analyses have been used to study the structure of a mouse VL30 gene and the molecular nature of VL30-related RNA which is induced upon the stimulation of cultured AKR mouse embryo cells with defined peptide growth factors. An integrated mouse VL30 gene was found to contain identical 601-base-pair long terminal repeats (LTRs) which were themselves terminated in short inverted repeats. The entire VL30 gene was flanked by a 4-base-pair direct repeat of cellular DNA. Thus, VL30 genes are structurally analogous to integrated forms of retrovirus proviruses and certain other classes of mobile genetic elements. The LTR sequence was found to contain putative promoter and polyadenylation signals and generally exhibited little sequence homology to murine leukemia virus proviral LTRs. Certain short regions of sequence conservation, however, were evident, including the inverted terminal repeat, LTR-adjacent regions corresponding to origins of murine leukemia virus proviral DNA synthesis, and a 36-base-pair direct repeat bearing homology to the 72-base-pair direct repeat (enhancer sequence) of the murine leukemia virus-related Moloney sarcoma virus. Upon mitogenic stimulation of quiescent cells with epidermal growth factor and insulin, a major 5.5-kilobase VL30-specific RNA complementary to both LTR and non-LTR sequences was rapidly induced. They conclude that a complete VL30 gene(s) is highly regulated by peptide growth factor binding to specific membrane receptors in these cells.
- Research Organization:
- Dept. of Cell Biology, Baylor College of Medicine, Houston, TX 77970
- OSTI ID:
- 5823358
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 3:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GROWTH FACTORS
DNA SEQUENCING
GENETIC MAPPING
ONCOGENES
GENE AMPLIFICATION
MUTAGENESIS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CELL MEMBRANES
CULTURE MEDIA
EMBRYONIC CELLS
HYBRIDIZATION
INSULIN
LEUKEMIA VIRUSES
MICE
MOLECULAR BIOLOGY
PHENOTYPE
RECEPTORS
SARCOMAS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSPOSONS
ANIMAL CELLS
ANIMALS
CELL CONSTITUENTS
DISEASES
GENES
HORMONES
MAMMALS
MAPPING
MEMBRANE PROTEINS
MEMBRANES
MICROORGANISMS
MITOGENS
NEOPLASMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PEPTIDE HORMONES
PROTEINS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
VERTEBRATES
VIRUSES
550400* - Genetics
550700 - Microbiology