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Title: Primary structure of the catalytic subunit of human DNA polymerase. delta. and chromosomal location of the gene

Abstract

The catalytic subunit of human DNA polymerase {delta} has been cloned by PCR using poly (A){sup +}RNA from HepG2 cells and primers designed from the amino acid sequence of regions highly conserved between bovine and yeast DNA polymerase {delta}. The human cDNA was 3,443 nucleotides in length and coded for a polypeptide of 1,107 amino acids. The enzyme was 94% identical to bovine DNA polymerase {delta} and contained the numerous highly conserved regions previously observed in the bovine and yeast enzymes. The human enzyme also contained two putative zinc-finger domains in the carboxyl end of the molecule, as well as a putative nuclear localization signal at the amino-terminal end. The gene coding for human DNA polymerase {delta} was localized to chromosome 19.

Authors:
;  [1]; ; ; ;  [2]
  1. Univ. of Washington, Seattle (United States)
  2. Univ. of Miami, FL (United States)
Publication Date:
OSTI Identifier:
5823149
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (United States)
Additional Journal Information:
Journal Volume: 88:24; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA POLYMERASES; MOLECULAR STRUCTURE; HUMAN CHROMOSOME 19; GENETIC MAPPING; RECOMBINANT DNA; DNA SEQUENCING; DNA-CLONING; LIVER CELLS; MAN; ANIMAL CELLS; ANIMALS; CHROMOSOMES; CLONING; DNA; DNA HYBRIDIZATION; ENZYMES; HUMAN CHROMOSOMES; HYBRIDIZATION; MAMMALS; MAPPING; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; PRIMATES; PROTEINS; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS; TRANSFERASES; VERTEBRATES; 550200* - Biochemistry

Citation Formats

Chung, D W, Davie, E W, Zhang, Jian, Tan, Chengkeat, So, A G, and Downey, K M. Primary structure of the catalytic subunit of human DNA polymerase. delta. and chromosomal location of the gene. United States: N. p., 1991. Web. doi:10.1073/pnas.88.24.11197.
Chung, D W, Davie, E W, Zhang, Jian, Tan, Chengkeat, So, A G, & Downey, K M. Primary structure of the catalytic subunit of human DNA polymerase. delta. and chromosomal location of the gene. United States. https://doi.org/10.1073/pnas.88.24.11197
Chung, D W, Davie, E W, Zhang, Jian, Tan, Chengkeat, So, A G, and Downey, K M. 1991. "Primary structure of the catalytic subunit of human DNA polymerase. delta. and chromosomal location of the gene". United States. https://doi.org/10.1073/pnas.88.24.11197.
@article{osti_5823149,
title = {Primary structure of the catalytic subunit of human DNA polymerase. delta. and chromosomal location of the gene},
author = {Chung, D W and Davie, E W and Zhang, Jian and Tan, Chengkeat and So, A G and Downey, K M},
abstractNote = {The catalytic subunit of human DNA polymerase {delta} has been cloned by PCR using poly (A){sup +}RNA from HepG2 cells and primers designed from the amino acid sequence of regions highly conserved between bovine and yeast DNA polymerase {delta}. The human cDNA was 3,443 nucleotides in length and coded for a polypeptide of 1,107 amino acids. The enzyme was 94% identical to bovine DNA polymerase {delta} and contained the numerous highly conserved regions previously observed in the bovine and yeast enzymes. The human enzyme also contained two putative zinc-finger domains in the carboxyl end of the molecule, as well as a putative nuclear localization signal at the amino-terminal end. The gene coding for human DNA polymerase {delta} was localized to chromosome 19.},
doi = {10.1073/pnas.88.24.11197},
url = {https://www.osti.gov/biblio/5823149}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 88:24,
place = {United States},
year = {Sun Dec 15 00:00:00 EST 1991},
month = {Sun Dec 15 00:00:00 EST 1991}
}