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Title: Diazepam binding inhibitor gene expression: Location in brain and peripheral tissues of rate

Abstract

Diazepam binding inhibitor (DBI), an endogenous 10-kDa polypeptide was isolated from rat and human brain by monitoring displacement of radioactive diazepam bound to specific recognition sites in brain synaptic and mitochondrial membranes. The cellular location of DBI mRNA was studied in rat brain and selected peripheral tissues by in situ hybridization histochemistry with a /sup 35/S-labeled single-stranded complementary RNA probe. DBI mRNA was heterogeneously distributed in rat brain, with particularly high levels in the area postrema, the cerebellar cortex, and ependyma of the third ventricle. Intermediate levels were found in the olfactory bulb, pontine nuclei, inferior colliculi, arcuate nucleus, and pineal gland. Relatively low but significant levels of silver grains were observed overlying many mesencephalic and telencephalic areas that have previously been shown to contain numerous DBI-immunoreactive neurons and a high density of central benzodiazepine receptors. In situ hybridizations also revealed high levels of DBI mRNA in the posterior lobe of the pituitary gland, liver, and germinal center of the white pulp of spleen, all tissues that are rich in peripheral benzodiazepine binding sites. The tissue-specific pattern of DBI gene expression described here could be exploited to further understand the physiological function of DBI in the brain and periphery.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Georgetown Univ. Medical Center, Washington, DC (USA)
OSTI Identifier:
5822713
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. USA; (United States)
Additional Journal Information:
Journal Volume: 85:18
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; AUTORADIOGRAPHY; ENZYME INHIBITORS; GENE REGULATION; MESSENGER-RNA; TISSUE DISTRIBUTION; CYTOCHEMISTRY; HYBRIDIZATION; LIGANDS; PITUITARY GLAND; POLYPEPTIDES; RECEPTORS; SULFUR 35; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOCHEMISTRY; BODY; CENTRAL NERVOUS SYSTEM; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; ENDOCRINE GLANDS; EVEN-ODD NUCLEI; GLANDS; ISOTOPES; LIGHT NUCLEI; MEMBRANE PROTEINS; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PEPTIDES; PROTEINS; RADIOISOTOPES; RNA; SULFUR ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Alho, H, Fremeau, Jr, R T, Tiedge, H, Wilcox, J, Bovolin, P, Brosius, J, Roberts, J L, and Costa, E. Diazepam binding inhibitor gene expression: Location in brain and peripheral tissues of rate. United States: N. p., 1988. Web. doi:10.1073/pnas.85.18.7018.
Alho, H, Fremeau, Jr, R T, Tiedge, H, Wilcox, J, Bovolin, P, Brosius, J, Roberts, J L, & Costa, E. Diazepam binding inhibitor gene expression: Location in brain and peripheral tissues of rate. United States. https://doi.org/10.1073/pnas.85.18.7018
Alho, H, Fremeau, Jr, R T, Tiedge, H, Wilcox, J, Bovolin, P, Brosius, J, Roberts, J L, and Costa, E. 1988. "Diazepam binding inhibitor gene expression: Location in brain and peripheral tissues of rate". United States. https://doi.org/10.1073/pnas.85.18.7018.
@article{osti_5822713,
title = {Diazepam binding inhibitor gene expression: Location in brain and peripheral tissues of rate},
author = {Alho, H and Fremeau, Jr, R T and Tiedge, H and Wilcox, J and Bovolin, P and Brosius, J and Roberts, J L and Costa, E},
abstractNote = {Diazepam binding inhibitor (DBI), an endogenous 10-kDa polypeptide was isolated from rat and human brain by monitoring displacement of radioactive diazepam bound to specific recognition sites in brain synaptic and mitochondrial membranes. The cellular location of DBI mRNA was studied in rat brain and selected peripheral tissues by in situ hybridization histochemistry with a /sup 35/S-labeled single-stranded complementary RNA probe. DBI mRNA was heterogeneously distributed in rat brain, with particularly high levels in the area postrema, the cerebellar cortex, and ependyma of the third ventricle. Intermediate levels were found in the olfactory bulb, pontine nuclei, inferior colliculi, arcuate nucleus, and pineal gland. Relatively low but significant levels of silver grains were observed overlying many mesencephalic and telencephalic areas that have previously been shown to contain numerous DBI-immunoreactive neurons and a high density of central benzodiazepine receptors. In situ hybridizations also revealed high levels of DBI mRNA in the posterior lobe of the pituitary gland, liver, and germinal center of the white pulp of spleen, all tissues that are rich in peripheral benzodiazepine binding sites. The tissue-specific pattern of DBI gene expression described here could be exploited to further understand the physiological function of DBI in the brain and periphery.},
doi = {10.1073/pnas.85.18.7018},
url = {https://www.osti.gov/biblio/5822713}, journal = {Proc. Natl. Acad. Sci. USA; (United States)},
number = ,
volume = 85:18,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 1988},
month = {Thu Sep 01 00:00:00 EDT 1988}
}