Molecular cloning of a cDNA coding biliary glycoprotein I: Primary structure of a glycoprotein immunologically crossreactive with carcinoembryonic antigen
The authors have isolated and sequenced four overlapping cDNA clones from a normal adult human colon library, which together gave the entire nucleotide sequence for biliary glycoprotein I (BGPI). BGPI is a member of the carcinoembryonic antigen (CEA) gene family, which is a subfamily in the immunoglobulin gene superfamily. The deduced amino acid sequence of the combined clones for BGP I revealed a 34-residue leader sequence followed by a 108-residue N-terminal domain, a 178-residue immunoglobulin-like domain, a 108-residue region specific to BGP I, a 24-residue transmembrane domain, and a 35-residue cytoplasmic domain. The nucleotide sequence of BGP I exhibited greater than 80% identity with CEA and nonspecific crossreacting antigen (NCA) in the leader peptide, N-terminal domain, and immunoglobulin-like domain. They propose that BGP I diverged from NCA by acquiring an immunoglobulin-like domain substantially different from the domains found in NCA or CEA and also a new cytoplasmic domain. The latter feature should result in a substantially different membrane anchorage mechanism of BGP I compared to CEA, which lacks the cytoplasmic domain and is anchored via a phosphatidylinositol-glycan structure. Protein structural analysis of BGP I isolated from human bile revealed a blocked N terminus, 129 amino acids of internal sequence that are in agreement with the translated cDNA sequence, and five glycosylation sites in the peptides sequenced.
- Research Organization:
- Beckman Research Institute of the City of Hope, Duarte, CA (USA)
- OSTI ID:
- 5822084
- Journal Information:
- Proc. Natl. Acad. Sci. USA; (United States), Vol. 85:18
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARCINOEMBRYONIC ANTIGEN
BIOLOGICAL EVOLUTION
GLUCOPROTEINS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA-CLONING
ADULTS
BILE
HYBRIDIZATION
LARGE INTESTINE
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
PHOSPHORUS 32
AGE GROUPS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOHYDRATES
CLONING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
GASTROINTESTINAL TRACT
GLYCOPROTEINS
INTESTINES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques