Origin of human chromosome 2: An ancestral telomere-telomere fusion

Journal Article · Tue Oct 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.20.9051· OSTI ID:5820919

Ijdo, J W; Baldini, A; Ward, D C; Reeders, S T; Wells, R A ^[1]

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1. Yale Univ., New Haven, CT (United States)

The authors identified two allelic genomic cosmids from human chromosome 2, c8.1 and c29B, each containing two inverted arrays of the vertebrate telomeric repeat in a head-to-head arrangement, 5{prime}(TTAGGG){sub n}-(CCCTAA){sub m}3{prime}. Sequences flanking this telomeric repeat are characteristic of present-day human pretelomeres. BAL-31 nuclease experiments with yeast artificial chromosome clones of human telomeres and fluorescence in situ hybridization reveal that sequences flanking these inverted repeats hybridize both to band 2q13 and to different, but overlapping, subsets of human chromosome ends. They conclude that the locus cloned in cosmids c8.1 and c29B is the relic of an ancient telomere-telomere fusion and marks the point at which two ancestral ape chromosomes fused to give rise to human chromosome 2.

• OSTI ID: 5820919
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:20; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COSMIDS
DNA SEQUENCING
HETEROCHROMOSOMES
BIOLOGICAL EVOLUTION
DNA HYBRIDIZATION
FLUORESCENCE
MAN
ANIMALS
CHROMOSOMES
HYBRIDIZATION
LUMINESCENCE
MAMMALS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550400* - Genetics