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Title: Molecular structure of the human argininosuccinate synthetase gene: Occurrence of alternative mRNA splicing

Abstract

The human genome contains one expressed argininosuccinate synthetase gene and ca. 14 pseudogenes that are dispersed to at least 11 human chromosomes. Eleven clones isolated from a human genomic DNA library were characterized extensively by restriction mapping, Southern blotting, and nucleotide sequencing. These 11 clones represent the entire expressed argininosuccinate synthetase gene that spans 63 kilobases and contains at least 13 exons. The expressed gene codes for two mRNAs that differ in their 5' untranslated sequences and arise by alternative splicing involving the inclusion or deletion of an entire exon. In normal human liver and cultured fibroblasts, the predominant mature argininosuccinate synthetase mRNA lacks sequences encoded by exon 2 in the expressed gene. In contrast, the predominant argininosuccinate synthetase mRNA in baboon liver contains exon 2 sequences. A transformed canavanine-resistant human cell line in which argininosuccinate synthetase activity is 180-fold higher than that in wild-type cells contains abundant amounts of both forms of the argininosuccinate synthetase mRNA. The mRNA lacking exon 2 sequences is the more abundant mRNA species in the canavanine-resistant cells. These observations show that splicing of the argininosuccinate synthetase mRNA is species specific in primates and varies among different human cell types.

Authors:
; ; ;
Publication Date:
Research Org.:
Dept. of Biological Chemistry, Univ. of Michigan Medical School, Ann Arbor, MI 48109
OSTI Identifier:
5810347
Resource Type:
Journal Article
Journal Name:
Mol. Cell. Biol.; (United States)
Additional Journal Information:
Journal Volume: 4:10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; LIGASES; GENES; GENETIC MAPPING; MOLECULAR STRUCTURE; RNA PROCESSING; SPECIES DIVERSITY; ARGINASE; ARGININE; BABOONS; CELL CULTURES; CHROMOSOME LOSSES; CHROMOSOMES; CONCANAVALIN; DNA SEQUENCING; ELECTROPHORESIS; GENE REGULATION; GENE REPRESSORS; GENETIC EFFECTS; LIVER; MAN; MESSENGER-RNA; MOLECULAR BIOLOGY; NUCLEOTIDES; SUCCINIC ACID; AGGLUTININS; AMIDASES; AMINO ACIDS; ANIMALS; ANTIBODIES; BIOLOGICAL EFFECTS; BODY; CARBOXYLIC ACIDS; DICARBOXYLIC ACIDS; DIGESTIVE SYSTEM; ENZYMES; GLANDS; HEMAGGLUTININS; HYDROLASES; LECTINS; LOSSES; MAMMALS; MAPPING; MONKEYS; NON-PEPTIDE C-N HYDROLASES; NUCLEIC ACIDS; NUCLEOPROTEINS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PRIMATES; PROTEINS; RNA; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550200* - Biochemistry

Citation Formats

Freytag, S O, Beaudet, A L, Bock, H G.O., and O'Brien, W E. Molecular structure of the human argininosuccinate synthetase gene: Occurrence of alternative mRNA splicing. United States: N. p., 1984. Web. doi:10.1128/MCB.4.10.1978.
Freytag, S O, Beaudet, A L, Bock, H G.O., & O'Brien, W E. Molecular structure of the human argininosuccinate synthetase gene: Occurrence of alternative mRNA splicing. United States. https://doi.org/10.1128/MCB.4.10.1978
Freytag, S O, Beaudet, A L, Bock, H G.O., and O'Brien, W E. 1984. "Molecular structure of the human argininosuccinate synthetase gene: Occurrence of alternative mRNA splicing". United States. https://doi.org/10.1128/MCB.4.10.1978.
@article{osti_5810347,
title = {Molecular structure of the human argininosuccinate synthetase gene: Occurrence of alternative mRNA splicing},
author = {Freytag, S O and Beaudet, A L and Bock, H G.O. and O'Brien, W E},
abstractNote = {The human genome contains one expressed argininosuccinate synthetase gene and ca. 14 pseudogenes that are dispersed to at least 11 human chromosomes. Eleven clones isolated from a human genomic DNA library were characterized extensively by restriction mapping, Southern blotting, and nucleotide sequencing. These 11 clones represent the entire expressed argininosuccinate synthetase gene that spans 63 kilobases and contains at least 13 exons. The expressed gene codes for two mRNAs that differ in their 5' untranslated sequences and arise by alternative splicing involving the inclusion or deletion of an entire exon. In normal human liver and cultured fibroblasts, the predominant mature argininosuccinate synthetase mRNA lacks sequences encoded by exon 2 in the expressed gene. In contrast, the predominant argininosuccinate synthetase mRNA in baboon liver contains exon 2 sequences. A transformed canavanine-resistant human cell line in which argininosuccinate synthetase activity is 180-fold higher than that in wild-type cells contains abundant amounts of both forms of the argininosuccinate synthetase mRNA. The mRNA lacking exon 2 sequences is the more abundant mRNA species in the canavanine-resistant cells. These observations show that splicing of the argininosuccinate synthetase mRNA is species specific in primates and varies among different human cell types.},
doi = {10.1128/MCB.4.10.1978},
url = {https://www.osti.gov/biblio/5810347}, journal = {Mol. Cell. Biol.; (United States)},
number = ,
volume = 4:10,
place = {United States},
year = {Mon Oct 01 00:00:00 EDT 1984},
month = {Mon Oct 01 00:00:00 EDT 1984}
}