Rifampin-stimulated uv resistance of phage lambda on Escherichia coli K12
The plaque survival of uv-irradiated phage lambda on excision-proficient E.coli strain AB1157 is greater if the host cells are exposed to rifampin for 10 minutes prior to infection. This repair is accompanied by little or no clear-plaque mutagenesis. Host cells uv-irradiated and incubated for 30 minutes in growth medium prior to treatment with rifampin show some Weigle-reactivation in addition to the repair stimulated by rifampin. Some clear-plaque Weigle-mutagenesis is also observed in the presence of rifampin: however, the amount is less than that seen in the absence of rifampin treatment. In contrast, the uv sensitivity of lambda on strain AB1886, an excision-repair deficient mutant, is unchanged by pre-treating the cells with rifampin, and no Weigle-reactivation is observed. These results suggest that repair of lambda on unirradiated cells in the presence of rifampin is an excision-dependent, error-free phenomenon. Since initiation of replication of lambda DNA requires RNA polymerase, and since rifampin blocks transcription by that polymerase, the effect of rifampin on the survival of lambda may be to delay phage replication, thereby allowing more time for excision repair to operate. 6 figures.
- Research Organization:
- Duke Univ., Durham, NC (USA). Dept. of Zoology
- DOE Contract Number:
- AS05-76EV03631
- OSTI ID:
- 5808090
- Report Number(s):
- CONF-8306264-1; ON: DE85008551
- Resource Relation:
- Conference: 11. annual meeting of the American Society for Photobiology, Madison, WI, USA, 26 Jun 1983
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIBIOTICS
RADIOSENSITIVITY EFFECTS
BACTERIOPHAGES
PHOTOREACTIVATION
BIOLOGICAL EFFECTS
DNA REPAIR
ESCHERICHIA COLI
EXPERIMENTAL DATA
MUTAGENESIS
PLAQUE FORMATION
ULTRAVIOLET RADIATION
ANTI-INFECTIVE AGENTS
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DATA
DRUGS
ELECTROMAGNETIC RADIATION
INFORMATION
MICROORGANISMS
NUMERICAL DATA
PARASITES
RADIATIONS
RECOVERY
REPAIR
VIRUSES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
560302 - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)