Multicellular tumor spheroid interactions with bone cells and bone

Journal Article · Tue Oct 01 00:00:00 EDT 1985 · Anat. Rec.; (United States)

DOI:https://doi.org/10.1002/ar.1092130202· OSTI ID:5805876

Wezeman, F H; Guzzino, K M; Waxler, B

In vitro coculture techniques were used to study HSDM1C1 murine fibrosarcoma multicellular tumor spheroid (HSDM1C1-MTS) interactions with mouse calvarial bone cells having osteoblastic characteristics and mouse bone explants. HSDM1C1-MTS attached to confluent bone cell monolayers and their attachment rate was quantified. HSDM1C1-MTS interaction with bone cells was further demonstrated by the release of /sup 3/H-deoxyuridine from prelabeled bone cells during coculture with multicellular tumor spheroids. HSDM1C1-MTS-induced cytotoxicity was mimicked by the addition of 10(-5) M prostaglandin E2 (PGE2) to /sup 3/H-deoxyuridine-labeled bone cells. The effects of low (10(-9) M) and high (10(-5) M) concentrations of PGE2 on bone cell proliferation were also studied. Higher concentrations of PGE2 inhibited bone cell proliferation. HSDM1C1-MTS resorbed living explants in the presence of indomethacin, suggesting that other tumor cell products may also participate in bone resorption. HSDM1C1-MTS caused direct bone resorption as measured by the significantly elevated release of /sup 45/Ca from prelabeled, devitalized calvaria. However, the growth of a confluent bone cell layer on devitalized, /sup 45/Ca-prelabeled calvaria resulted in a significant reduction in the amount of /sup 45/Ca released subsequent to the seeding of HSDM1C1-MTS onto the explants. Bone cells at the bone surface may act as a barrier against invasion and tumor cell-mediated bone resorption. Violation of this cellular barrier is achieved, in part, by tumor cell products.

• OSTI ID: 5805876
• Journal Information: Anat. Rec.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BONE CELLS
CELL PROLIFERATION
PROSTAGLANDINS
BIOLOGICAL EFFECTS
DEOXYURIDINE
FIBROSARCOMAS
INHIBITION
MICE
MITOTIC INDEX
SKELETON
SKULL
TRACER TECHNIQUES
TRANSPLANTS
TRITIUM COMPOUNDS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BODY
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
RODENTS
SARCOMAS
SOMATIC CELLS
URACILS
VERTEBRATES
550901* - Pathology- Tracer Techniques