Effects of UV radiation on plaque formation of Herpes Simplex Type 1 on C3H/10T/sub 1/2/ monolayers
HSV-1 plaque-forming ability and plaque sizes were assayed on C3H/10T/sub 1/2/ monolayers as a function of the following variables: (1) pretreatment dose with UV light at different times before inoculation; (2) pretreatment dose with cycloheximide 24 hours before inoculation; and (3) population density of monolayers at time of inoculation. Irradiated virus exhibited smaller plaques than in controls (small plaque effect, SPE). The capacity of the cells was found to increase substantially with pretreatment with UV light or cycloheximide (capacity enhancement effect, CE); these agents also produced larger plaques than on controls (the large plaque effect, LPE). No significant UV light-enhanced reactivation (ER) was observed; thus, UV light-induced ER and LPE were not correlated in this system. Pretreatment with cycloheximide, however, seemed to produce a moderate level of ER.
- Research Organization:
- Pennsylvania State Univ., University Park (USA)
- OSTI ID:
- 5804855
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
VIRUSES
CYCLOHEXIMIDE
HERPES SIMPLEX
IN VITRO
PLAQUE FORMATION
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
INFECTIOUS DISEASES
MICROORGANISMS
PARASITES
PESTICIDES
RADIATION EFFECTS
RADIATIONS
SKIN DISEASES
SOMATIC CELLS
VIRAL DISEASES
560121* - Radiation Effects on Cells- External Source- (-1987)
560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)